. Asiatic herpetological research. Reptiles -- Asia Periodicals; Amphibians -- Asia Periodicals. Vol. 3, p. 118 Asiatic Herpetological Research April 1990 100. 10 20 30 time(min) 40 50 60 FIG. 3. RP-HPLC of a-chymotryptic digests of the dodecapeptide. Column: uBondapak Cis mm. Mobile phase: A= CF3COOH B=60% CH3CN in A. Linear gradient elution from 0-60%B , 40 min, at ml/min. Detected at UV 220 nm, aufs. Peak (*) is the unreacted dodecapeptide. established as in Fig. 2. The peak 33 had the same properties with the peak 32 in the amino acid compositions and sequence ana
. Asiatic herpetological research. Reptiles -- Asia Periodicals; Amphibians -- Asia Periodicals. Vol. 3, p. 118 Asiatic Herpetological Research April 1990 100. 10 20 30 time(min) 40 50 60 FIG. 3. RP-HPLC of a-chymotryptic digests of the dodecapeptide. Column: uBondapak Cis mm. Mobile phase: A= CF3COOH B=60% CH3CN in A. Linear gradient elution from 0-60%B , 40 min, at ml/min. Detected at UV 220 nm, aufs. Peak (*) is the unreacted dodecapeptide. established as in Fig. 2. The peak 33 had the same properties with the peak 32 in the amino acid compositions and sequence analysis, respectively. We do not know the structural differences between the two peaks. Based on the amino acid analysis, the yield of the pure dodecapeptide (including peak 32 and 33 in Fig. 1) was estimated to be at least nmol starting from g of fresh skin, according to that the rate of recovery of all above isolation steps was 10%. The dodecapeptide was inactive in GPI test, and its activity awaits to be established by assay methods other than those used in the present screening. Discussion The dodecapeptide described above is a completely novel peptide. The peptide may be a member of a new peptide family, and it should be regarded as the biochemical characteristic of Oreolalax pingii in taxonomy. Furthermore, the C-terminal portion of the dodecapeptide has some homologies to the mammalian Leu- enkephalin (Hughes et al. 1975) as shown below: The dodecapeptide: Gly-Leu-Val-Ser-Asp- Leu-Met-Try-Gly-Ile-Gly-Leu-NH2 Leu-enkephalin: Try-Gly-Gly-Phe-Leu In amphibian skin peptide research, it. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Asiatic Herpetological Research Society; Chung-kuo liang chi pa hsing tung wu hs©eh hui. Berkeley, Calif. : Asiatic Herpetological Research Society : Chinese Society f
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