. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 374 JAMES S. McDANIEL 100 09 -t—> GO >« o X 60 20. 15 45 Minutes 75 FIGURE 5. Effectiveness of diluted media on excystment. Representative experiments shown. Conditions: 30 minutes in pepsin (pH 2} at 37° C, remove to; O—O trypsin plus sodium taurocholate (NaT), • —• trypsin plus NaT, D—D trypsin plus NaT, A—A trypsin plus NaT. rate of excystment increased with higher concentrations, hut bile salt slightly repressed the rate after 15 minutes (Fig. 4). Optimum excy
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 374 JAMES S. McDANIEL 100 09 -t—> GO >« o X 60 20. 15 45 Minutes 75 FIGURE 5. Effectiveness of diluted media on excystment. Representative experiments shown. Conditions: 30 minutes in pepsin (pH 2} at 37° C, remove to; O—O trypsin plus sodium taurocholate (NaT), • —• trypsin plus NaT, D—D trypsin plus NaT, A—A trypsin plus NaT. rate of excystment increased with higher concentrations, hut bile salt slightly repressed the rate after 15 minutes (Fig. 4). Optimum excystment in trypsin-hile salt after pepsin treatment was attained with 1% trypsin plus sodium taurocholate pretreated for 30 minutes with 1% pepsin. However, excellent excystment (65-75%) occurred also with 10-fold and greater dilutions of these substances (Fig. 5). DISCUSSION Stimuli necessary for excystment of the metacercariae of trematodes are found in the host's intestine. However, escape from enclosing membranes entails re- sponses which differ among the various species. The process is apparently more closely related to that of cestodes and acanthocephalans than that of nematodes (see Rothman, 1959; Rogers, 1962; Graff and Kitzman, 1965). Faust and Khaw (1925, 1927) found that the metacercaria of Clonorchis. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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