. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 12 - I I l I l ii. D pH FIGURE 6. Plots of velocity (V, color change/30 min assay) of azoalbumin hydrolysis (lower graph) and per cent inactivation (%l, upper graph) versus pH of tlie incubation medium ( pH unit increments) for a- and /3-chymotrypsin with and without a 15 min pre- incubation with Hyinowlepis diminuta. Symbols in lower and upper graphs as in Figures 1 and 4, respectively. Lines were fitted by inspection. did not activate the inactive zymogen (A and B, Table IV). It may be noted that E minus F of Ta
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 12 - I I l I l ii. D pH FIGURE 6. Plots of velocity (V, color change/30 min assay) of azoalbumin hydrolysis (lower graph) and per cent inactivation (%l, upper graph) versus pH of tlie incubation medium ( pH unit increments) for a- and /3-chymotrypsin with and without a 15 min pre- incubation with Hyinowlepis diminuta. Symbols in lower and upper graphs as in Figures 1 and 4, respectively. Lines were fitted by inspection. did not activate the inactive zymogen (A and B, Table IV). It may be noted that E minus F of Table IV shows that trypsin itself is inactivated by the intact worm as reported by Pappas and Read (1972). When the effect of pH on the inactivation of a- and /J-chymotrypsin was examined, the results were as shown in Figure 6. Between pH and , pH clearly had a negligible effect on enzyme inactivation. There is a suggestion that there may be decreased inactivation at pH DISCUSSION Some of the results of this study resemble the findings of our earlier study deal- ing with the effects of H. diinhutta on pancreatic trypsin (Pappas and Read, 1972). That is, inactivation of a- and /3-chymotrypsin is dependent on the period of ex- posure of the worms to the enzyme ; inactivation appears to be irreversible ; inactiva- tion is not dependent upon the nature of the substrate nor is it blocked by polyions. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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