. Biological structure and function; proceedings. Biochemistry; Cytology. mM p-FPhe. Fk;. 6. " Set-back" against concentration of ^-FPhe in inorganic medium. The exposure is always for 20 min. Crosses: Division i—Cells immersed at 25 min. after EH. Circles: Division 2—Cells immersed at 55 min. after EH. A complicating factor in the experiments described is in the complexity of the growth medium. For cells (" washed cells ") in the inorganic medium the extracellular pool of amino acids is nil and the sensitivity of the cells to amino acid analogues is much increased. For the
. Biological structure and function; proceedings. Biochemistry; Cytology. mM p-FPhe. Fk;. 6. " Set-back" against concentration of ^-FPhe in inorganic medium. The exposure is always for 20 min. Crosses: Division i—Cells immersed at 25 min. after EH. Circles: Division 2—Cells immersed at 55 min. after EH. A complicating factor in the experiments described is in the complexity of the growth medium. For cells (" washed cells ") in the inorganic medium the extracellular pool of amino acids is nil and the sensitivity of the cells to amino acid analogues is much increased. For the washed cells we have. 60 90 Minutes of exposure to p-FPhe. Ekj. 7. " Set-back" (ordinate) as a function of the duration (abscissa) for which exposure is made to o-8 mM p-FPhe in inorganic medium. The curves are for different times after EH of beginning treatment with /)-FPhe. EH is the time when the last temperature shock ends. been able to define combinations of concentrations (Fig. 6) of/)-FPhe and of exposure times (Fig. 7) w^hich give maximal delaying efi^ects. Figure 6 show^s the relation between response of the washed cells and concentration of ^-FPhe added at 25 (crosses) and 55 (circles) min. after EH. Exposure is for a standard time of 20 min. The analogue is removed by four washings with the inorganic medium. Washing is only complete if I mM DL-phenylalanine is added. So this was done. The response is represented by the "set-back", w^hich is equal to the delay of the division, minus the time for which the cells were in contact with the inhibitor. The crosses represent "set-backs" for division i, the circles relate similarly to division 2. For both divisions maximal effects are obtained with o-16 mM. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS Internat
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