. Pathological technique; a practical manual for workers in pathological histology and bacteriology. water, 10 ; Concentrated sulphuric acid, 2 drops; 5 per cent, oxalic acid, I drop. 3. Wash out quickly in distilled water. 4. Absolute alcohol. 5. Xylol. 6. Xylol balsam. It is recommended to place the section for a few minutesbefore staining in the 1 : 10,000 caustic-potash solution. Schutzs Method.— 1. Stain twenty-four hours in equalparts of concentrated alcoholic solution of methylene-blueand caustic potash, 1 : 10,000. PATHOGENIC BACTERIA AND FUNGI. 319 2. Wash in acidified water. 3. 5
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. water, 10 ; Concentrated sulphuric acid, 2 drops; 5 per cent, oxalic acid, I drop. 3. Wash out quickly in distilled water. 4. Absolute alcohol. 5. Xylol. 6. Xylol balsam. It is recommended to place the section for a few minutesbefore staining in the 1 : 10,000 caustic-potash solution. Schutzs Method.— 1. Stain twenty-four hours in equalparts of concentrated alcoholic solution of methylene-blueand caustic potash, 1 : 10,000. PATHOGENIC BACTERIA AND FUNGI. 319 2. Wash in acidified water. 3. 50 per cent, alcohol for five minutes. 4. Absolute alcohol for five minutes. 5. Xylol. 6. Xylol balsam. Noniewiczs Method.—1. Stain in Lofflers methylene-blue solution two to five minutes. 2. Wash in water. 3. Decolorize one to five seconds in \ per cent, acetic acid, 75 parts; \ per cent, aqueous solution of tropeolin, 25 4. Wash in water. 5. Dehydrate section on slide with filter-paper; then inthe air ; finally, over small flame. 6. Clear by dropping xylol on it repeatedly. 7. Xylol Fig. 76.—Bacillus of chancroid in smear preparation from pus (Lincoln Davis ;photo by L. S. Brown). Bacillus of Chancroid (Bacillus of Ducrey).—In smears from the lesions the bacilli appear as short, round-ended rods, about 1,5 p. long and fi thick, occurringcharacteristically but not always in chains. The middleportion of the rods does not stain so deeply as the bacilli are decolorized by Grams method of staining,and are not motile. The following description of the cul- 320 PA THOL 0GICAL TECHNIQ UE. tural peculiarities of the chancroid bacillus are based on theobservations of Dr. Lincoln Davis in the Laboratory of theMassachusetts General Hospital. The bacillus does not grow on the ordinary culture-media, but may be cultivated in blood or in media contain-ing one-third its volume of blood. It is essential that theblood be fresh. In tubes containing blood, or a mixtur
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