. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 488 J. A STRAND, J. T. CUMMINS AND B. E. VAUGHAN lected were accurately identified in the field. Verification of closely related forms was achieved through standard histological technique. A fixing fluid consisting of 1 g. chromic acid, 1 ml. propionic acid in 90 cc. sea water proved to be satis- factory for most marine algae. Delafield's or Harris' hematoxylin was a suitable stain for most preparations; an appropriate counter stain of erythrosin B, orange G () or fast-green (Johansen, 1951, pp. 359, 361) was also used.
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 488 J. A STRAND, J. T. CUMMINS AND B. E. VAUGHAN lected were accurately identified in the field. Verification of closely related forms was achieved through standard histological technique. A fixing fluid consisting of 1 g. chromic acid, 1 ml. propionic acid in 90 cc. sea water proved to be satis- factory for most marine algae. Delafield's or Harris' hematoxylin was a suitable stain for most preparations; an appropriate counter stain of erythrosin B, orange G () or fast-green (Johansen, 1951, pp. 359, 361) was also used. Transplants, prior to placement into aquaria, were rinsed in fresh sea water and pesticide, using Lindane (hexachloro-cyclohexane), 5 ppm. for 15 minutes, NRDL 389-66 SUPPLY TO OTHER AQUARIA. OTHER AQUARIA FIGURE 1. Flow diagram for recirculation aquarium system: temperature 16 ± 1° C.; salinity 25 ± 3%c; total filtration from 4 to 10/x; pH ± ; recirculation 190 ; sea water replacement 4% per day. Details described in text. to kill resident herbivorous gastropods and polychaetes. Polarographic measure- ments at the surface of Ulva after 24 hours exposure indicate that Lindane at concentrations of 650 ppm. did not appreciably inhibit oxygen evolution (report in preparation). Of eight requirements to obtain optimal growth, five were adjusted empirically: (1) micronutrient enrichment, (2) CO2 as a carbon source, (3) salinity and pH stabilization, (4) light source of suitable intensity and spectral quality, and (5) agitation and aeration. Additional requirements were necessitated due to the re- circulation of sea water within the system: (6) nonmetallic construction, (7) filtra- tion and sterilization, and (8) sea water replenishment. A schematic diagram of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the orig
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
