Archive image from page 88 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 Endonnclease Digestion for Cosmids Positive for STS D12S950. The positive cosmids for D12S950 were tested with Eco RI, Eco RI and Bam HI, and Bgi II endonucleases. Of the eleven cosmids initially determined as strongly labeling during hybridization there were several that did not digest well as seen by the very faint or nonexistent banding (FIGURE 27). Only those eight, which showed distinct banding patterns, were digested with Bgl II: c64
Archive image from page 88 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 Endonnclease Digestion for Cosmids Positive for STS D12S950. The positive cosmids for D12S950 were tested with Eco RI, Eco RI and Bam HI, and Bgi II endonucleases. Of the eleven cosmids initially determined as strongly labeling during hybridization there were several that did not digest well as seen by the very faint or nonexistent banding (FIGURE 27). Only those eight, which showed distinct banding patterns, were digested with Bgl II: c64A5, cl33A5, cI64G4, cl67Fl, cl89C3, c203F5, c205G9. and c221116. Many of these digestions did not produce clear, distinct banding when electrophoresed and, as such, a clear, distinct could not be determined. Figure 27. Restriction Enzyme Digestion with Eco RI and Bam HI of Cosmids Positive for Marker DI2S950 as electrophoresed on a agarose gel. For gel I: Lane 2 contains (ig I KB ladder. Lanes 3 and 10 have |.ig I Hind III DNA, Lane 4 has c64A5, Lane 5 has c66G2. Lane 6 has cl33A5, Lane7hascl64G4, Lane 8 has cl67FI. and Lane 9 has cl70G6. For gel 2: Lane 2 contains Hg 1KB ladder. Lanes 3 and 9 have ?. Hind III DNA, Lane 4 has cl75FI I, Lane 5 hascl89C3, Lane 6 has c203F5, Lane 7 has c205G9, and Lane 8 has c221H6. Endomtclease Digestion for Cosmids Positive for STS D12SI987. No endonuclease digestions were carried out upon these cosmids. Further testing would use the same technique as the digestions carried out upon STS D12S1914 and D12S950 in hopes of producing a cosmid map of the region surrounding the marker. 43
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