. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. WATER RESERVOIR EFFECTS OF HYPOXIA ON CRASSOSTREA EXPERIMENTAL CHAMBER SERIES Uow 267. AIRSTONE \ WATER INTAKE Figure 1. The experimental apparatus. Four chambers of one treatment are shown. Flasks of seawater were bubbled with air, a mix of oxygen and nitrogen, or nitrogen. The equilibrated seawater was pumped through chambers containing settlement substrate and pediveliger larvae or juveniles of the oyster Crassostrea virginica. Flow-through chambers were immersed in a circulating water bath of 25°C. (Not drawn to scale.)


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. WATER RESERVOIR EFFECTS OF HYPOXIA ON CRASSOSTREA EXPERIMENTAL CHAMBER SERIES Uow 267. AIRSTONE \ WATER INTAKE Figure 1. The experimental apparatus. Four chambers of one treatment are shown. Flasks of seawater were bubbled with air, a mix of oxygen and nitrogen, or nitrogen. The equilibrated seawater was pumped through chambers containing settlement substrate and pediveliger larvae or juveniles of the oyster Crassostrea virginica. Flow-through chambers were immersed in a circulating water bath of 25°C. (Not drawn to scale.) and 38% at 48 h. After 48 h, hypoxic treatments had no further settlement. In anoxic treatments, settlement was 4% at 24 h, with no subsequent settlement. At 24 h, anoxic and normoxic treatment means were significantly different (P < ), and at 48 h, the anoxic treatment mean was significantly different (P < ) from both the hypoxic and normoxic treatment means. At 72 and 96 h, all three treatment means were significantly different (P < ) from each other. Juvenile growth Regressions of log transformed juvenile oyster growth data from normoxic and hypoxic treatments were linear and significant. The regression coefficients of the normoxic and hypoxic treatments were not significantly different; however, the regression elevations were significantly dif- ferent (P & W 20 ,- i V ;'/ : f ' v/ n ^ i > m 24 48 72 96 Hours exposure Figure 2. Relation between percentage settlement of oyster (Cras- sostrea virginica) pediveliger larvae and duration of normoxic ( mg O2 r1), hypoxic ( mg O2 1~'), and anoxic (< mg O2 P1) treat- ments. (Means + SD; normoxia n = 5; hypoxia n = 3; anoxia n = 3.) o O) "55 D) o 2 0 x — Normoxia A Hypoxia 24 48 72 96 120 144 Hours post settlement Figure 3. Log of growth of Crassostrea virginica juveniles (initial shell height 290 ^m) in normoxic ( mg O2 1~'), hypoxic ( mg O2 1"'). and anoxic (< mg O2


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology