. Bulletin. Science; Natural history; Natural history. Fiiiurc 3. Analytical ultracentrifuge patterns using Schlieren optics of different preparations of adult gray whale postanal sac fluid. The centrifugation pattern is moving from left to right. A. Native, un- diluted sac fluid, pH ; 72 min. B, Sac fluid, diluted 1:.*! in Tris buffer, pH ; 26 min. C, Fraction I ( G-150), reconstituted in Tris buffer, pH ; 31 min. D, Fraction II (Sephadex G-150), re- constituted in Tris buffer, pH ; 39 min. question through the identification of specific antigenic components. The sediment
. Bulletin. Science; Natural history; Natural history. Fiiiurc 3. Analytical ultracentrifuge patterns using Schlieren optics of different preparations of adult gray whale postanal sac fluid. The centrifugation pattern is moving from left to right. A. Native, un- diluted sac fluid, pH ; 72 min. B, Sac fluid, diluted 1:.*! in Tris buffer, pH ; 26 min. C, Fraction I ( G-150), reconstituted in Tris buffer, pH ; 31 min. D, Fraction II (Sephadex G-150), re- constituted in Tris buffer, pH ; 39 min. question through the identification of specific antigenic components. The sedimentation coefficients of these two main components show some variance dependent upon the conditions of assay. The higher molecu- lar weight component, fraction I, averages S whereas fraction II has an approximate sedimen- tation coefficient of Fraction II however is not absolutely homogeneous, as suggested by gel electrophoresis (Fig. 4) and the peak spread one obtains on columns and gels, or in the ultracen- trifuge. Both the colorimetric analyses (Table 2) and the staining procedures used in acrylamide gel electrophoresis (Fig, 4) indicate that the higher molecular weight constituent (fraction I) is a protein-polysaccharide, and more specifically a • t .r I jA MB A B C Figure 4. Acrylamide gel electrophoretic patterns of the postanal sac fluid, using differential staining. Tops of the gels are to the right; cathode to the left, a. Native sac fluid; b. Fraction I; Sephadex G-150; c, Fraction II; Sephadex G-150. A, Alcian blue; B, Coomassie blue; C, PAS. glycoprotein. The presence of sialic acid and fucose, in the presence of uronic acid also suggest that this particular glycoprotein is unique, as these three sugars have never been reported together in a glycoprotein molecule (Schmid, 1972). Frac- tion II stains only with Coomassie blue and does not contain sialic or uronic acids, or amino sugars. By lowering the pH to one obtains a precipi- tate at that isoelectric
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