. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ALDH-LIKE PROTEIN IN A SYMBIOTIC ORGAN 319. Figure 7. Diagrammatic reconstruction of a light organ from a mm juvenile (estimated to be between 7 and 10 days old), illustrating the area where ALDH-like protein is first detected in the ciliated duct epithelium. Arrow points to darkened nuclei, representing cells within the ciliated duct epithelium which contain colloidal gold spheres, im- mediately adjacent to non-ciliated epithelium, lining the bacteria-con- taining crypts. All other epithelial cells, represented by clear
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ALDH-LIKE PROTEIN IN A SYMBIOTIC ORGAN 319. Figure 7. Diagrammatic reconstruction of a light organ from a mm juvenile (estimated to be between 7 and 10 days old), illustrating the area where ALDH-like protein is first detected in the ciliated duct epithelium. Arrow points to darkened nuclei, representing cells within the ciliated duct epithelium which contain colloidal gold spheres, im- mediately adjacent to non-ciliated epithelium, lining the bacteria-con- taining crypts. All other epithelial cells, represented by clear nuclei, both in the bacteria-containing inner area and in the ciliated duct, lack colloidal gold spheres, b, bacteria-containing crypts; d, ciliated duct epithelium; i, ink sac; mes, mesodermal tissue, the site of the presumptive lens; p, the pore connecting the ciliated duct with the outside of the light organ; and r, reflector. Bar = 50 /urn. We have tested light organ lenses extensively for ALDH activity and found none (Montgomery and McFall-Ngai, 1992), but we have detected very low activities in whole light organs (Montgomery, unpub. data). Activity stains (see above) in the light organ might be a useful technique to further explore the function of the ALDH-like protein species in the ciliated duct epithelium. A positive stain in this tissue would suggest an enzymatic role for this protein. Unlike lens cells, ciliated duct epithelium comes in di- rect contact with symbiotic bacteria and possibly other bacteria that enter the light organ through the pores. Some ALDH isozymes function in the detoxification of various biogenic aldehydes that are produced under certain con- ditions, such as during lipid peroxidation (Holmes el a/., 1989) and oxidative stress (Jedziniak el ai. 1987; Abedinia el ai, 1990). Such conditions have been proposed for sev- eral symbioses (Dykens and Shick, 1982; Blum and Fri- dovich, 1984). It is possible that an enzymatically active ALDH, and thus a
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