. Pathological technique; a practical manual for workers in pathological histology and bacteriology. Fig. 140.—Photomicrographs of living filariae sanguinis hominis; X 250, a, tromhydrocele fluid; b, from blood (after Lothrop and Pratt). be examined during the resting hours of the patient, asat night for day-workers and during the day for night-workers. Permanent specimens can be made by fixing 408 PATHOLOGICAL TECHNIQUE. ordinary cover-slip preparations of the blood or chylousfluid by heat or by the use of a saturated solution of corro-sive sublimate, and staining for a few seconds with Loffl
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. Fig. 140.—Photomicrographs of living filariae sanguinis hominis; X 250, a, tromhydrocele fluid; b, from blood (after Lothrop and Pratt). be examined during the resting hours of the patient, asat night for day-workers and during the day for night-workers. Permanent specimens can be made by fixing 408 PATHOLOGICAL TECHNIQUE. ordinary cover-slip preparations of the blood or chylousfluid by heat or by the use of a saturated solution of corro-sive sublimate, and staining for a few seconds with Lofflersmethylene-blue or with a 2 per cent, aqueous solution ofthionin. In the case of suspected hookworm disease the feces are tobe examined microscopically for the eggs of the parasite, or. Fig. 141.—Two eggs of the hookworm in feces, each containing several embry-onic cells; X 375. (On C. L. Overlander.) for the adult worms after the administration of an anthelmin-tic. The finding of the eggs may be facilitated by mixingthe feces with a nine-tenths saturated aqueous solution ofsodium chloride in a test tube and examining microscopicallythe superficial portions of the fluid, where any eggs presentwill accumulate because their specific gravity is less thanthat of the fluid. This procedure was first recommended byBass. In fresh feces, eggs containing several embryoniccells are most common. (See Fig. 141.) Trichinellse (Figs. 142, 143) are obtained from the freshmuscle by means of teasing. A quick method is to squeezesmall bits of tissue between two slides and examine with alow power. Pieces of muscle nearest the insertion of thetendon are chosen from the diaphragm or from the musclesof the jaws. Encapsulated and calcified trichinellae are clearedup by means of acids. ANI
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