. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EVOLUTIONARY TEMPERATURE ADAPTATION 413 30-. 10 if. 20 25 30 35 TEMPERATURE ( C) Figure 1. The effects of temperature on Kd of ['H]CHA. Filled square: Rtillus rattus: open square: Gallim ilomeslicus; open inverse triangle: Epinephelus lul\'ii.\, open triangle: Sebastolobus alaxcumi^. open circle: Macrounis ht'rgla.\- tilled triangle: S. ultin'lis: filled circle: Coryphacnoidcs nipt'slns; tilled inverse triangle: Antimora mstraia. The standard error for each value is approximately 12% of the Kj. Each point represents at least
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EVOLUTIONARY TEMPERATURE ADAPTATION 413 30-. 10 if. 20 25 30 35 TEMPERATURE ( C) Figure 1. The effects of temperature on Kd of ['H]CHA. Filled square: Rtillus rattus: open square: Gallim ilomeslicus; open inverse triangle: Epinephelus lul\'ii.\, open triangle: Sebastolobus alaxcumi^. open circle: Macrounis ht'rgla.\- tilled triangle: S. ultin'lis: filled circle: Coryphacnoidcs nipt'slns; tilled inverse triangle: Antimora mstraia. The standard error for each value is approximately 12% of the Kj. Each point represents at least two determinations. apparent dissociation constant, AG° the standard free energy change, AH" the standard enthalpy change, AS" the standard entropy change. R the gas constant ( cal mol"' K"'), and T the absolute temperature. Binding enthalpies were determined from van't Ho ft' plots of In Ka versus the reciprocal absolute temperature using the integrated van't Hoft"equation: In Ka = -AH°/RT + AS"/R. Ka values were calculated using the experimentally deter- mined Kj at each temperature. The data were fit using a least squares linear regression. For the [3H]CH A competition experiments conducted at 5°C with A. rostruia brain membranes, IC\0 values and slope factors were determined using the nonlinear least squares curve-fitting program LIGAND (Munson and Robard. 1980). Dissociation constants (K,) were deter- mined using the equation: K, = ICs0/[l +([L]/Kd)] where [L] is the total [3H]CHA concentration employed. Kd is the apparent dissociation constant of [3H]CHA and is the concentration of inhibitor resulting in 50% inhibition of the specific binding (Cheng and Prusoff 1973). Results Effects of temperature on Kdand B,,,,,x The effects of temperature at pH on K^ of [3H]- CHA values differed among the eight species studied (Fig. 1). Agonist binding in chicken and rat brain mem- branes was the most sensitive to temperature changes: Kj
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
