. A text-book of mycology and plant pathology . Plant diseases; Fungi in agriculture; Plant diseases; Fungi. 6l2 LABORATORY EXERCISES the whole is sterilized. To each flask 2 of a 2 per cent, solution of ammonium sulphate is subsequently added. The temperature for incubation is 30°C (Fig. 216). This organism can be successfully grown on silicate jelly. As silicate jelly is difficult to make it is optional for the students to attempt its manufacture. For reference the method is given.' Pot Experiments with Nitrogen Fixation.^—Since the experiments of Hellriegel and Wilfarth and other exper
. A text-book of mycology and plant pathology . Plant diseases; Fungi in agriculture; Plant diseases; Fungi. 6l2 LABORATORY EXERCISES the whole is sterilized. To each flask 2 of a 2 per cent, solution of ammonium sulphate is subsequently added. The temperature for incubation is 30°C (Fig. 216). This organism can be successfully grown on silicate jelly. As silicate jelly is difficult to make it is optional for the students to attempt its manufacture. For reference the method is given.' Pot Experiments with Nitrogen Fixation.^—Since the experiments of Hellriegel and Wilfarth and other experimenters,..it has been known that certain bacteria. Fig. 216.—Double-walled copper incubator constructed with non-conducting materials, with water gauge and openings for insertion of thermometer and thermo- iti Padded outer door of copper, inner door of glass. (Fig. 22, p. 46, Schneider, pharmaceutical Bacteriology, 1912.) (Bacillus radicicola, etc.) have the power of fixing free atmospheric nitrogen, when dey enter the roots of leguminous plants with the formation of root nodules. The (formation of these nodules can be followed in a series of experiments. ' It is optional of course for the teacher to omit these rather difficult exercises entirely. If followed by the student or class, a useful work to consult, in connection with Lesson 17 is Smith, Erwin F.: Bacteria in Relation to Plant Diseases, I : 36-39. 2 An important paper on the culture and isolation of Bacillus radicicola is by Hakkison, F. C. and Barlow, B.: The Nodule Organism of the Leguminosse—Its Isolation, Cultivation and Commercial Application. Centralblatt fiir Bakteriologie, Parasitenkunde und Infektionskrankheiten, 19, Abt 2, 1907: 264-272, 426-440, pis. 9. Consult for other details Lipman, J. G. and Brown, P. E.: A Laboratory Guide in Soil Bacteriology, Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appe
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