. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 32 FREDERICK CRESCITELLI trifuged out. After decanting the supernatant fluid, the cells were washed in cold sea water and again centrifuged out. This process was repeated three or four times after which the cells were hemolvzed in cold distilled water. The solution of j pigment after filtration and dilution to ahout one hundred ml. with cold distilled water was examined spectroscopically. Results and discussion The five curves (Fig. 1) obtained from five different sea cucumbers (Ciicn- iiuirio ininiata) give an indication of
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 32 FREDERICK CRESCITELLI trifuged out. After decanting the supernatant fluid, the cells were washed in cold sea water and again centrifuged out. This process was repeated three or four times after which the cells were hemolvzed in cold distilled water. The solution of j pigment after filtration and dilution to ahout one hundred ml. with cold distilled water was examined spectroscopically. Results and discussion The five curves (Fig. 1) obtained from five different sea cucumbers (Ciicn- iiuirio ininiata) give an indication of the reproducibility of the spectral curve and show that the location of a point of maximum or minimum can be checked within 2 m/t. These curves, which were obtained from well-aerated solutions of the pig- ment, show two maxima; one 580-581 niju., the other at 544-545 in/x. The point D I I 51O 5EO SJO 540 SSO S6O 57O 58O S9O 6OO 61O 62O 63O FIGURE 1. The absorption spectral curves of the pigment from five different sea cucumbers (Cttcitnwria iniiiiata). The density (log In/I) is plotted as ordinates against the wave length, in mM. The individual graphs are plotted on a multiple ordinate scale to avoid crowding. The zero ordinate for each curve is indicated by the corresponding letter. Each plotted point repre- sents the mean of 3-5 spectrometric readings. of minimum absorption between these two maxima is at 564-565 m^.. A compari- son of the spectral curves of the pigments, in the oxidized state, from all three species is shown in Figure 2. It is evident that all three pigments show two maxima, but the maxima for Eudistylia and for Molpadia are shifted toward the red end of the spectrum when compared with the Cucumaria data, the shift being greater in the case of Molpadia. The position of the maximum and minimum points for all the curves that were obtained from all three organisms are listed in Table I. Reduction of the pigment by means of sodium hyposulphite res
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
