Annals of tropical medicine and parasitology . In the Luangwa Valley, 3,202 flies were fedin twenty-nine batches, and Trypanosoma rhodesiense was isolatedin six of the experiments, giving a ratio of i infective fly to 534,whereas on the Congo-Zambesi watershed, 5,250 freshly-caughtGlossina morsitans were fed in groups on forty-one monkeys, withfour positive results—i infective fly to 1,312. As tsetse flies andgame are about equally numerous at Nawalia and Ngoa, and as thedisease was presumably introduced into the two localities, whichare less than seventy miles apart, about the same time, it a


Annals of tropical medicine and parasitology . In the Luangwa Valley, 3,202 flies were fedin twenty-nine batches, and Trypanosoma rhodesiense was isolatedin six of the experiments, giving a ratio of i infective fly to 534,whereas on the Congo-Zambesi watershed, 5,250 freshly-caughtGlossina morsitans were fed in groups on forty-one monkeys, withfour positive results—i infective fly to 1,312. As tsetse flies andgame are about equally numerous at Nawalia and Ngoa, and as thedisease was presumably introduced into the two localities, whichare less than seventy miles apart, about the same time, it appearsto us that the only essential difference which can account for thefact that the percentage of infective wild flies at Nawalia is twoand a half times as great as at Ngoa is the difference in theclimatic conditions. It will be seen from Tables i and 2 that thetemperatures experienced on the Congo-Zambesi watershed duringMay, June and July, are very much lower than those at Nawalia Annals Trap. Med. & ParasitoL, Vol. VII PLATE XV11. Fig. I. Feeding Glossina morsitans on experimental animals.


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