. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 560 KENNEY, BELAMARICH AND SHEPRO 100-1. TIME (sec) FIGURE 7. Typical experiment showing the effect of EDTA on the release of aggregation promoting material from amebocytes. Control amebocyte pellet represents amebocytes with- drawn in saline. EDTA amebocyte pellet represents amebocytes withdrawn in 75 HIM final EDTA. At 0 time (4 min after puncture) supernatants of both pellets contained nil inactive plasma + ml saline, and did not produce significant aggregation of amebocytes in EDTA (X); aggregation in saline (con
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 560 KENNEY, BELAMARICH AND SHEPRO 100-1. TIME (sec) FIGURE 7. Typical experiment showing the effect of EDTA on the release of aggregation promoting material from amebocytes. Control amebocyte pellet represents amebocytes with- drawn in saline. EDTA amebocyte pellet represents amebocytes withdrawn in 75 HIM final EDTA. At 0 time (4 min after puncture) supernatants of both pellets contained nil inactive plasma + ml saline, and did not produce significant aggregation of amebocytes in EDTA (X); aggregation in saline (control) (•); amebocytes in 17 ± 6 mM EDTA (O). Amebocytes in EDTA added to 1:20 diluted supernatants of: control amebocyte pellet at 1' (A); control amebocyte pellet at 10' (•); control amebocyte pellet at 20' (4); EDTA amebocyte pellet at 1' (A) ; EDTA amebocyte pellet at 10' (D) ; EDTA amebocyte pellet at 20' (0) ; EDTA amebocyte pellet at 30' (0). periods of 1, 10, 20, and 30 min, at which times the supernatants are removed, checked microscopically to insure the absence of cells or cell fragments, and assayed for ability to restore aggregation to amebocytes in EDTA. At each time interval, the activity of the supernatants of EDTA treated pellets is compared with the control, saline—plasma supernatant of amebocytes originally withdrawn in a 1: 3 ratio with saline. The results of a representative experiment (Fig. 7) show that at any given time the supernatant of the control amebocyte pellet is significantly more active in producing aggregation of amebocytes in EDTA than the super- natants of the EDTA treated amebocyte pellet. Reversing activity is present in. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass.
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
