Nanopore sequencing of DNA. The Minion nanopore sequencer is designed to determine the order in which nucleotides occur on a strand of DNA (deoxyribon
Nanopore sequencing of DNA. The Minion nanopore sequencer is designed to determine the order in which nucleotides occur on a strand of DNA (deoxyribonucleic acid). A nanopore is a hole in a membrane with a diameter of around 1 nanometre. When a nanopore is immersed in a conducting fluid and a voltage is applied across it, ions are conducted through it creating a current. When a strand of DNA passes through the nanopore, each nucleotide obstructs the nanopore to a different, characteristic degree, creating a characteristic change in the current. The current changes represent a direct reading of the DNA sequence. Photographed at Genoscope National Sequencing Centre, University of Evry, France.
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Photo credit: © PATRICE LATRON/EURELIOS/LOOK AT SCIENCES/SCIENCE PHOTO LIBRARY / Alamy / Afripics
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