. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LOBSTER ECDYSTEROID METABOLISM 479 0. E o 1/1 V vc. t> Q- 20 n 10 Hemolymph Urine. 40 50 60 Retention Time (min) Figure 4. Reverse phase-HPLC-scintillation spectromelric analyses ofhemolymph, urine, and fecal [3H]-ecdysteroids. Samples were obtained from a stage C4 lobster 96 h after injection of pH]-ecdysone. Separation conditions are described in Figure 1. The retention times of authentic 20-hydroxyecdysonoic acid epimers (20EA), 20,26-dihydroxyecdysone (), 20-hydroxyecdysone (20E), ecdysone (E), ponasterone A (P)
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LOBSTER ECDYSTEROID METABOLISM 479 0. E o 1/1 V vc. t> Q- 20 n 10 Hemolymph Urine. 40 50 60 Retention Time (min) Figure 4. Reverse phase-HPLC-scintillation spectromelric analyses ofhemolymph, urine, and fecal [3H]-ecdysteroids. Samples were obtained from a stage C4 lobster 96 h after injection of pH]-ecdysone. Separation conditions are described in Figure 1. The retention times of authentic 20-hydroxyecdysonoic acid epimers (20EA), 20,26-dihydroxyecdysone (), 20-hydroxyecdysone (20E), ecdysone (E), ponasterone A (P), and 22,25-dideoxyecdysone (triol. T) are shown. Two highly polar ec- dysteroid products, which include conjugates and non-enzyme-hydro- lyzable metabolites, are labeled as HP1 and HP2. with a retention time similar to that of 22,25-dideoxyec- dysone (triol, T). Only small percentages of [3H]-20E, ec- dysone, and P were ever found in the feces of any molt stage. Metabolite profiles for stage D2-D, are shown in Figure 5. The major hemolymph ecdysteroid at this stage was 20E, with smaller amounts of HP1, HP2, , and ecdysone. The urinary profile is also shown. Fecal [3H]- ecdysteroids were >99% apolar products. Hydrolysis of the fecal apolar material yielded a number of products (Fig. 6). The profiles of fecal ecdysteroid conjugates varied according to molt stage. Figure 6 shows the profile of a premolt stage D, lobster fecal sample 10 days post-injec- tion; at this time, 20E was the major hemolymph metab- olite. Hydrolysis yielded a large percentage of 20E, and smaller amounts of HP1, ecdysone, P, T, and unhydro- lyzed apolar components. During intermolt stage C, hy- drolysis of fecal apolar ecdysteroids resulted in a higher percentage of free HP (data not shown). The uptake and metabolism of [3H]-ecdysone injected into hemolymph was studied in juvenile lobsters in in- termolt stage C (Table II). By 1 h after injection, only 33% of the radiolabel remained in the hemolym
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
