A Reference handbook of the medical sciences embracing the entire range of scientific and practical medicine and allied science . tion of the medium and ex-])ulsion of the air, tlie tubes are sealed in the it is desired to obtain colonies, the tube can be con-verted into an Esmarch roll tube, as shown in This principle has been adapted in various ways forthe of obtaining plate cultures. Kitasato em-ployed a flat bottle, having a tube fused at the lowerend. The dilutions were made in the ordinary tubes,after which the material was poured into these flasks,which were
A Reference handbook of the medical sciences embracing the entire range of scientific and practical medicine and allied science . tion of the medium and ex-])ulsion of the air, tlie tubes are sealed in the it is desired to obtain colonies, the tube can be con-verted into an Esmarch roll tube, as shown in This principle has been adapted in various ways forthe of obtaining plate cultures. Kitasato em-ployed a flat bottle, having a tube fused at the lowerend. The dilutions were made in the ordinary tubes,after which the material was poured into these flasks,which were connected in series and liydrogen passedthrough. Finally the ends were sealed by fusing in theflame, while the neck of each flask was closed with aclamped rubber lube. Several modifications of this bottlehave been made, but they are little used, since methods 589 Bn<leriuloi;l<aITecliuiqiie. REFERENCE HANDBOOK OP THE MEDICAL SCIENCES. Avcre soon pciffptctl whereby it was possible to makePclii ])liiUs in hvdrosicn. One of tlie eiirlicstiitlempts in this direction was thatof BlUchcr. wlio made use of a funnel whicli was. Firi. 5093.—Botkins Apparatus for Plate Culture of weiglited with lead and inverted over the plates in alarger dish. Air was excluded by means of glycerinwaUr. Hesse inverted a glass vessel in a circular troughfilled with mercury. Liborins used a copper bell jar ,which was compressed against a rubber gasket by means Iof set screws; others made use of bell jars inverted upona ground glass surface. In many respects the Rotkinapparatus is useful. It is shown in Fig. 5093. It con-sists of a metal rack on which are placed the Petri is set in a large outer dish which contains about aninch of liquid petroleum. A bell jar is inverted over thestand. The inflow and outflow tubes are of rubber stilf-ened b_y a copper wire on the inside. After the hydrogenhas been passed for a sufficient length of time, tiie tubesare withdrawn and the apparatu
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