. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 156 JOHN BUCK AND MARGARET KEISTER (Buck, Keister and Specht, 1953). Ordinarily this "purge" began when the flasks were flushed with ,, thus being mainly lost in the equilibration period, but occa- sionally it was delayed as much as 15 minutes, permitting direct measure (Fig. 9). From such records it was found that purges differ from normal CO., bursts in being spread over 31 minutes (mean for 16 purges) and in involving over twice as much CO., as a normal burst of the same pupa, even when the flushing is perfor
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 156 JOHN BUCK AND MARGARET KEISTER (Buck, Keister and Specht, 1953). Ordinarily this "purge" began when the flasks were flushed with ,, thus being mainly lost in the equilibration period, but occa- sionally it was delayed as much as 15 minutes, permitting direct measure (Fig. 9). From such records it was found that purges differ from normal CO., bursts in being spread over 31 minutes (mean for 16 purges) and in involving over twice as much CO., as a normal burst of the same pupa, even when the flushing is performed immediately after a normal burst in air has occurred. A similar prolonged bleeding I20r LU 100 UJ o 01 LU Q. BURST VOLUME ..».. CYCLE LENGTH i i i i i I 1 1 1 1 1 1 1 I I I i 1 1 I I I I I I 1 1 1. 0 FIGURE 8. Cycle changes in Ions experiments; 30°. The hurst plot was obtained by first plotting, for each pupa, each individual burst volume (as % of mean hurst volume for the whole experiment for that pupa) at the time of its occurrence and connecting the points with straight lines. Then the intercepts at the selected 3^, 6^, 9 Mi. otc. hour ordinates with each individual pupal plot were averaged and plotted. The cycle length plot was obtained similarly, plotting the individual cycle lengths (as % of pupal mean) at the time of occurrence of the burst ending the particular interburst period. All pupae gave three or more- hursts, and there were 10 pupae in the 13% group and 22 in the control group. off of CO., was seen after spiracular intubation. We interpret this to mean that there is normally present in the tissue and blood at all times a large reserve of CO2 and CO, derivatives, and that in a burst the spiracles close again before all this stored CO., is released. In an anaerobic environment, or after intubation, on the other hand, the spiracles are forced to stay open, allowing additional CO2 to diffuse out. Thus the first interburst period in air subsequent to a N ..-ind
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