. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 182 P. F. SCHOLANDER, C. L. CLAFF AND S. L. SVEINSSON cysts was immeasurable by this method. In order to effect excystment the cyst was placed for five minutes in sterile hay tea and then transferred several times in sterile 1/50 dilute Ringer solution in which it was run. Hay tea is a better medium for excystment but was found too difficult to maintain sterile. After the activation by hay tea there is a steady increase in oxygen consumption, and at the moment the organism breaks through the cyst there is a definite but ofte
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 182 P. F. SCHOLANDER, C. L. CLAFF AND S. L. SVEINSSON cysts was immeasurable by this method. In order to effect excystment the cyst was placed for five minutes in sterile hay tea and then transferred several times in sterile 1/50 dilute Ringer solution in which it was run. Hay tea is a better medium for excystment but was found too difficult to maintain sterile. After the activation by hay tea there is a steady increase in oxygen consumption, and at the moment the organism breaks through the cyst there is a definite but often quite small rise. It is conceivable that this rise is merely due to the elimina- /"/L (-MILLIONTH OF MM3) 16,00 /400 1200- 1000- £00 £,00 VOO 200- BftESSLAUA. J L 0 10 20 30 10 50 60 10 20 30 HO SO WJ MINUTE5 FIGURE 2. Oxygen consumption at excystment and encystment in single individuals of Brcsslaua insidiatrix. tion of the shell, which would act as a limiting factor for the oxygen diffusion at the high metabolic rate of the fully developed cell. In Figure 2 three runs are given showing the reverse effect on the oxygen con- sumption when the cells are encysting. We wish to express our gratitude to Dr. A. Baird Hastings for much helpful advice during the work and to the officers of the William F. Milton Fund for sup- porting this Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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