. Pathological technique; a practical manual for workers in pathological histology and bacteriology. ons of the aniline dyes are Gram-Weigert stain gives a brilliant picture. Thesame methods can be employed as for tubercle bacilli if adifferential stain is desired. A method recommended byElexner will be found very useful. 1. Stain in alum-hematoxylin so as to get a sharp nuclearstain. 2. Wash in water. 3. Carbol-fuchsin two to five minutes steaming, or thirtyto sixty minutes cold. 4. Water. 5. Treat on the slide with iodin solution one-half to oneminute. 6. Water. 7. Blot; clear
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. ons of the aniline dyes are Gram-Weigert stain gives a brilliant picture. Thesame methods can be employed as for tubercle bacilli if adifferential stain is desired. A method recommended byElexner will be found very useful. 1. Stain in alum-hematoxylin so as to get a sharp nuclearstain. 2. Wash in water. 3. Carbol-fuchsin two to five minutes steaming, or thirtyto sixty minutes cold. 4. Water. 5. Treat on the slide with iodin solution one-half to oneminute. 6. Water. 7. Blot; clear and differentiate in aniline oil. 8. Xylol; balsam. Baumgarten gives the following . differential stain forleprosy bacilli: 1. Stain six to seven minutes in a dilute solution of fuch-sin (5 drops of a concentrated alcoholic solution to a watch-glass of water). 2. Discharge one-quarter minute in nitric acid alcohol(nitric acid I, alcohol 10). 3. Wash in water. PATHOGENIC BACTERIA AND FUNGI. 355 4. Contrast-stain in a saturated aqueous solution of meth-ylene-blue. 5. Alcohol. 6. Xylol. 7.
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Keywords: ., bookcentury1900, bookdecade1910, bookidpath, booksubjectpathology
