Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . or suspected material is introduced, after which alayer of sterile cotton is laid over the wound and a thick coating of col-lodion applied; in a fewminutes this will dry;the animal is then liber-ated and kept with anuninoculated animalknown as the injectionof bacteria, suspendedin sterile water or bouil-lon, may be resorted toas directed for intraperi-toneal inoculation. 2. Intraperitoneal in-jections are made by preparing the animal as before. A syringe graduated
Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . or suspected material is introduced, after which alayer of sterile cotton is laid over the wound and a thick coating of col-lodion applied; in a fewminutes this will dry;the animal is then liber-ated and kept with anuninoculated animalknown as the injectionof bacteria, suspendedin sterile water or bouil-lon, may be resorted toas directed for intraperi-toneal inoculation. 2. Intraperitoneal in-jections are made by preparing the animal as before. A syringe graduatedin cubic centimeters is necessary for this method. The skin of the ab-dominal wall—the site of the operation—is prepared as previouslydirected, grasped by an attendant, and raised as a fold, into which theneedle is introduced and forced onward into the peritoneum, thenpartly withdrawn to make sure it is free in the peritoneal syringe is now attached, a given quantity of bouillon culture isintroduced, and the wound is sealed. Rosenau^ has greatly im-^ Hygienic Laboratory Bull. No. 19, 44S.—Kichs Svrtxge for Hypodermic, lNTRAPERiTi>xFAL, andOTHER Injection Methods for Inoculating A^^MALs?, theCapacity Should Be About Two Cubic Centimeters. BACTi;kU)L(.)eiic T];ciinr. 925 ])roveil the KulIi syringe, and describes a method foe .i>>,uiiii^ .nc uratedosage. ?. Intravenous iiiocitlatioii is made directly into a vein; the mostconvenient veins are tliose of the rabbits ear. Tlie overlying skin isl>repared as already directed, and an incision is made down ui)on, butnot into, the vein; wlien the vessel is thoroughly exposed, the needleof the hypodermic syringe is thrust into the vein in the line of the blood-eurrent, ami the contents of the syringe slowly injected. Cireat caremust be used to see that no air enters the vein, and that no clumps orother solid material that might cause embolism are thrown into the blood. -/. I)iocnlatioii i)ito the Anterior
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