. Elements of applied microscopy. A text-book for beginners. Microscopy. i6 ELEMENTS Oh APPLIED MICROSCOPY. site surfaces of the lenses according to certain known relations. Such lenses are designated as aplanatic. There still remained one serious limitation to the in- crease of the power of magnification of the compound microscope. In all its ^^arious refractions there is a loss of light, and with the small objective lenses of great curva- ture, necessary for high magnification, it is difficult to get a sufficient illumination for clear vision. Furthermore, it has been shown by Abbe and other


. Elements of applied microscopy. A text-book for beginners. Microscopy. i6 ELEMENTS Oh APPLIED MICROSCOPY. site surfaces of the lenses according to certain known relations. Such lenses are designated as aplanatic. There still remained one serious limitation to the in- crease of the power of magnification of the compound microscope. In all its ^^arious refractions there is a loss of light, and with the small objective lenses of great curva- ture, necessary for high magnification, it is difficult to get a sufficient illumination for clear vision. Furthermore, it has been shown by Abbe and others that the rays which extend from a point toward the periphery of a lens are of prime importance in the detection of the finer structure of objects. If the outer zones of rays are lost, no image of very minute details can be formed; and this is just what occurs in the ordi- nary compound microscope when there is air between the objective and the specimen to be examined, as is shown in the right half of Fig. 14. If, on the other. Fig. 14.—Effect of Homogeneous Immersion. (After Hager-Mez.) hand, some substance like cedar-oil, which has the same refractive index as glass, be placed between the lens and the cover-shp which covers the specimen, the rays will. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Winslow, C. -E. A. (Charles-Edward Amory), 1877-1957. New York, J. Wiley; London, Chapman & Hall


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