. Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . Fig. 2. Unusually potent phagocvte 2 minutes after injection of colloidal gold. Lumen on right; perisinusoidal space on left. One large branched cleft filled with gold particles is seen communicating with lumen. At A a gold-containing cleft communicates with the perisinusoidal space. At B a rounded profile probably represents a spherical, membrane- enclosed inclusion. Maunification 32,000. cause of their greater content of particles, many of which were contained in spherical or spheroidal mem- brane-enclosed incl
. Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . Fig. 2. Unusually potent phagocvte 2 minutes after injection of colloidal gold. Lumen on right; perisinusoidal space on left. One large branched cleft filled with gold particles is seen communicating with lumen. At A a gold-containing cleft communicates with the perisinusoidal space. At B a rounded profile probably represents a spherical, membrane- enclosed inclusion. Maunification 32,000. cause of their greater content of particles, many of which were contained in spherical or spheroidal mem- brane-enclosed inclusions of various sizes. In some instances the membrane of an inclusion was seen to be double. The most remarkable observation made on this material was on certain particle-con- taining cleft-like spaces (these may or may not have been present in cells at 15 seconds following injec- tion, but were not seen). These spaces ran a highly irregular, sometimes branching, sometimes exagge- ratedly serpentine course, suggesting that the section was passing through an area of complicated inter- digitation of two neighboring cells. However, in a few instances a strong impression was gained that the whole formation was intracellular; , that the cleft represented an extremely complicated infolding of the surface membrane of a single ceil. Some clefts that were obviously intracellular were seen appar- ently breaking up into small spheroidal inclusions. some of which contained particles. Again, it was impossible to decide whether such clefts, if intracellu- lar, pre-existed or formed in response to the presence of foreign particles. The small amount of particulate material (often in widely separated groups) in some clefts suggested that the clefts had not been formed in response to the presence of the particles. It was further noted that a certain amount of phagocytosis of material in the clefts passing from lumen to perisinusoidal space had taken place, as evidenced by spheroidal inclus
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