. The Biological bulletin. Biology; Zoology; Marine biology. BLOOD CELLS AND EXCRETION IN ASCIDIANS 251 nificance to note here that SuHma (1914) made a quantitative analysis of the purine content of the body tissues of Cynthia nucrocosvAus {Microcosmus vulgaris). From his analyses he found a mean value of about per cent uric acid and per cent other purine bases (cal- culated as xanthine). Within the living excretory cells the granules have certain char- acteristic optical qualities. By oblique or reflected light they shine in a dark background. This is a characteristic found in purine


. The Biological bulletin. Biology; Zoology; Marine biology. BLOOD CELLS AND EXCRETION IN ASCIDIANS 251 nificance to note here that SuHma (1914) made a quantitative analysis of the purine content of the body tissues of Cynthia nucrocosvAus {Microcosmus vulgaris). From his analyses he found a mean value of about per cent uric acid and per cent other purine bases (cal- culated as xanthine). Within the living excretory cells the granules have certain char- acteristic optical qualities. By oblique or reflected light they shine in a dark background. This is a characteristic found in purine containing cells of other animals. Millot (1923), who made a detailed study of the guanophores of the lower vertebrates, found that crystals of guanine in the cells took on a brilliant aspect against a dark background. The reflecting granules in ascidian blood cells are one color by transmitted light and another color by reflected light. Such two-color granules, which may vary in size from the limits of visibility with the highest powers of the microscope up to concretions several microns in diameter,. Fig. L Excretory cells of ascidian blood, a, b, and c are vesicular cells with reflecting concretions from the blood of Polyandrocarpa tincta; b is a young stage, c? is a vesicular cell with reflecting granules from the blood of Pyura vittata. I have found m Claveliim picta, C. ohlonga, Distaplia hermudensis of the family Polycitoridse; in Symplegma viride, Polycarpa ohtecta, and Polyandrocarpa tincta of the family Styelidse; in Pyura vittata of the family Pyuridae; in Botryllus schlosseri and Botrylloides niger of the family Botryllidae. In Ecteinascidia turbinata Herdman and in E. conklini minuta Berrill of the family Ascidiidas there are similar cells, but I could not see the intravacuolar granules in the living cells because of the presence of a film at the surface of the vacuoles. This film, like the granules in other species, was brown by transmitted light and white or yell


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