. Cytology. Cytology. Cr Manometer Flotation nnediunn Zero line Diver. Flotation medium (lithium chloride) Gas space total vol. about 24X 2X Holtfreter solution Tissue Glass tail "U Fine adjustment -|J Coarse adjustment Figure 11-39. Schematic Diagram of Cartesian Diver Micromanometer. (A) Diver vessel with diver suspended in flotation medium (concentrated lithium chloride solution). The diver vessel is connected to a water manom- eter, the pressure in which can be adjusted by a coarse and fine syringe mechanism (bottom). (B) Details of Cartesian diver. The tissue is placed in 2 X (1 X =
. Cytology. Cytology. Cr Manometer Flotation nnediunn Zero line Diver. Flotation medium (lithium chloride) Gas space total vol. about 24X 2X Holtfreter solution Tissue Glass tail "U Fine adjustment -|J Coarse adjustment Figure 11-39. Schematic Diagram of Cartesian Diver Micromanometer. (A) Diver vessel with diver suspended in flotation medium (concentrated lithium chloride solution). The diver vessel is connected to a water manom- eter, the pressure in which can be adjusted by a coarse and fine syringe mechanism (bottom). (B) Details of Cartesian diver. The tissue is placed in 2 X (1 X = 1 cu. mm.) of physiological medium at the bottom of the diver bulb which has a glass tail to maintain the diver in a vertical position. The neck is partly occupied by a drop of oil, so placed that there is a small bubble of air between the oil and the flotation medium. A reading is taken by bring- ing the diver to a temporary standstill at the zero line and noting the pressure level in the manometer. (From Boell, E. J., Needham, J., and Peters, V., 1939. "Morphogenesis and Metabolism: Studies with the Cartesian Diver Ultramicromanometer. 1. Anaerobic Glycolysis of the Regions of the Am- phibian Gastrula," Proc. Roy. Microscop. Soc. {London), 127, Fig. 1, p. 326.) SURVEY OF CYTOLOGICAL TECHNIQUES / 263. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Wilson, G. B. (George Bernard), 1914-; Morrison, John H. (John Herbert), 1927-. New York, Reinhold
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