The Journal of biological chemistry . 294 215 362 268 395 304 534 345 567 433 533 TABLE (E) and Substrate (A) Concentrations for Actions Given in Table I. ExperimentNo. E (arbitraryunits). A (in tenths of milli- equivalents of glyceryl triacetate). Initial pH. Time in hrs. to reachpH to 45(A) * 4 45(B) * 4 46(A) 14 (not tested before). 46(B) 14 47(A-Tu) 47(B-Tu) 47(B-M) 34
The Journal of biological chemistry . 294 215 362 268 395 304 534 345 567 433 533 TABLE (E) and Substrate (A) Concentrations for Actions Given in Table I. ExperimentNo. E (arbitraryunits). A (in tenths of milli- equivalents of glyceryl triacetate). Initial pH. Time in hrs. to reachpH to 45(A) * 4 45(B) * 4 46(A) 14 (not tested before). 46(B) 14 47(A-Tu) 47(B-Tu) 47(B-M) * Concentrations Concentrations Concentrations comparable. Sugiura, Noyes, and Falk 907 (B-Tu), (A-Tu), and (B-M) after an initial constancy extendingover about 40 time units. The most obvious explanation of thisdecrease, assuming the kinetic equation to interpret correctly thechemical equation for each experiment, is a progressive decreasein enzyme concentration due to spontaneous inactivation or to. 280 320 360 Time 520 Fig. 1. Time-action results of two rat tumor extracts, Nos. and 46,on glyceryl triacetate. Abscissa values, \ hour units; ordinate values,tenths of milli-equivalents of ester hydrolyzed. Concentration of tumor,three times as great in the B series as in the A series; concentration of ester,If times as great in the B series as in the A series. the action of the products of the reaction. The initial constancyof K in Experiments 47 (B-Tu), (A-Tu), and (B-M) wherethe hydrogen ion concentration did not change, followed by thefalling off in K indicates that the increasing concentration of theproducts of the reaction caused the decrease in K. The continuous 908 Kinetics of Lipase Actions decrease of K in Experiments 45 and 46 may then be ascribed to achange in hydrogen ion concentration from pH to with theaccompanying less favorable conditions for actions (1) dm-ing thefirst period, followed then by the increasing concentra
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbiochem, bookyear1905
