. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . HOMYCETES 235 Permanent Specimens.—Remove fat with chloroform, then place material informic acid and heat to boiling (two or three minutes). Remove acid by wash-ing m distilled water, stain with Lofflers methylene blue. Wash, dehydratein absolute alcohol, clear in xylol and mount in balsam. Sabourauds media are best for growth of the organism (p. 110). Upon thismedium within half a dozen days septate mycehal threads with clilamydo-spores (p. 27) are seen. Reddish or brown pigment sometimes develops
. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . HOMYCETES 235 Permanent Specimens.—Remove fat with chloroform, then place material informic acid and heat to boiling (two or three minutes). Remove acid by wash-ing m distilled water, stain with Lofflers methylene blue. Wash, dehydratein absolute alcohol, clear in xylol and mount in balsam. Sabourauds media are best for growth of the organism (p. 110). Upon thismedium within half a dozen days septate mycehal threads with clilamydo-spores (p. 27) are seen. Reddish or brown pigment sometimes develops. According to Sabouraud, whose conclusions are based on an extensiveseries of microscopic examinations of cases of tinea in man and animals,of cultivation in artificial media, and of inoculation on man and animals,there are two distinct types of the fungi causing ringworm in man—one with small spores (2/i to d/j) which are known as microspora, andone with large spores (7m to 8m) which are called megahspora. Theydiffer in their mode of growth on artificial media and in their pathological. Fig. 91.—Aspergillus fumigatus. Gelatin culture. Spread stained with gentian violet500 : 1. (From Itzerott and Niemann.) effects on the human skin and its appendages. The small spored typeis the common fungus of Tinea tonsurans of children, especially of thosecases which are rebellious to treatment, and its special seat of growthis in the substance of the hair. T. megalosporon (Fig. 92) is essentiallythe fungus of ringworm of the beard and of the smooth part of the skin;the prognosis as regards treatment is good. One-third of the cases ofT. tonsurans of children are due to megalospora. The spores of are contained in a mycelium; but this is not visible, thespores appearing irregularly piled up like zooglea masses; and, growingoutside, they form a dense sheath around the hair. The spores of are always contained in distinct mycelium filaments, whichmay either b
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