. Laboratory work in bacteriology. Bacteriology. BOUILLON, AGAE AND MILK. 23T While the filter is still hot, and the pump active, the well sedimented agar (p. 236) is slowly poured on the cen- ter of the filter. The liquid passes through as rapidly as it strikes the surface. The impurities are brought last upon the filter. If the filtrate is not perfectly clear, it can be passed through the filter a second or a third time. The operation of filtration requires but a few minutes. The resulting filtrate, when filled into tubes and sterilized, is usually wholly free from a de- posit, and is perfec
. Laboratory work in bacteriology. Bacteriology. BOUILLON, AGAE AND MILK. 23T While the filter is still hot, and the pump active, the well sedimented agar (p. 236) is slowly poured on the cen- ter of the filter. The liquid passes through as rapidly as it strikes the surface. The impurities are brought last upon the filter. If the filtrate is not perfectly clear, it can be passed through the filter a second or a third time. The operation of filtration requires but a few minutes. The resulting filtrate, when filled into tubes and sterilized, is usually wholly free from a de- posit, and is perfectly transparent. At most, a few fioccules will separate during the subsequent steriliza- tion. In order to facili- tate the filtration through paper many workers prefer to make a 1 or lj4 per cent, solution of agar. While it is true that such solutions filter more readily, yet this advantage is more than offset by the softer consistency of the agar. The two per cent, agar prepared as above is hard, and though it is not filtered through paper, it is, nevertheless, perfectly clear. The above method of making agar would seem to be long and tedious, but in reality, it is no more difficult than the preparation of gelatin. While the pump is very useful, in the above filtration, it is by no means necessary. In the method as described, the agar is not added ta the albuminous meat extract, but to the ready-made bquil-. FiG. 35. Apparatus for filtering agar {F. G. N.)-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Novy, Frederick George, 1864- . Ann Arbor [Mich. ] G. Wahr
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