. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 450 M. SUSSMAN Thirteen of these determinations were made using seven different quantities of Nc-4 cells/drop ranging from X 103 to X 104. Figure 1 shows the re- sults of three such experiments. Each point in the curves represents the mean of 30 replicate drops. In the absence of agg-53 cells, X 103 Nc-4 produced no centers (curve III of Fig. 1), as a result of the extremely low population density. (From the average radius of the drops and the number of cells deposited, the density was estimated to be less than 5
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 450 M. SUSSMAN Thirteen of these determinations were made using seven different quantities of Nc-4 cells/drop ranging from X 103 to X 104. Figure 1 shows the re- sults of three such experiments. Each point in the curves represents the mean of 30 replicate drops. In the absence of agg-53 cells, X 103 Nc-4 produced no centers (curve III of Fig. 1), as a result of the extremely low population density. (From the average radius of the drops and the number of cells deposited, the density was estimated to be less than 50 ) Alone, X 103 Nc-4 formed a mean of cen- ters/drop ; X 103 Nc-4 produced centers/drop. Control suspensions of agg-53 cells were never observed to give rise to aggregate centers. Addition of -O i The relation between the number of centers formed and the number of agg-53A present in mixtures with Wild Type NC-4. n m 60 No. agg -53A/drop x 10 -5 FIGURE 2. Curve I refers to mixtures containing X 103 wild type cells/sample with various numbers of agg-53A. Curve II refers to mixtures containing X 103 wild type cells/sample and Curve III, X 103. agg-53 cells to the wild type suspensions, however, markedly increased the num- bers of centers formed. The presence of between 2 and 3 X 105 agg-53 cells re- sulted in maximal center formation whose absolute value depended upon the number of wild type. Thus for 8 X 103 Nc-4, a plateau value of centers/drop was reached. For 5 X 10a Nc-4 the value was and for X 103 Nc-4 it was One center was produced for approximately 1000 wild type cells present. A similar set of experiments was performed using mixtures of Nc-4 and agg- 53A. Figure 2 summarizes the results of three determinations. The curves are in qualitative agreement with those of Figure 1 except that significantly different plateaus were reached for the same numbers of Nc-4 employed. Using agg-53A as the test
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