. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 236 M. FAINZ1LBER ET AL. = i->dle (OCD 29\im ,OCD 150 pm) . naie (OCD 311 (jm) !ermolt ). 246 Time (hours) Figure 1. The incorporation rate of leucine into protein secreted by in vitro incubated subepidermal adipose tissue (SAT) in Peneatts semi- .'iitlcalns. Tissues were removed from a male, and previtellogenic and vitellogenic females. Results were calculated for whole SAT. The mean ± for four replicates are given in each case. The average oocyte diameter (OCD) in ovaries is indicated (in ^m) for each female. stage
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 236 M. FAINZ1LBER ET AL. = i->dle (OCD 29\im ,OCD 150 pm) . naie (OCD 311 (jm) !ermolt ). 246 Time (hours) Figure 1. The incorporation rate of leucine into protein secreted by in vitro incubated subepidermal adipose tissue (SAT) in Peneatts semi- .'iitlcalns. Tissues were removed from a male, and previtellogenic and vitellogenic females. Results were calculated for whole SAT. The mean ± for four replicates are given in each case. The average oocyte diameter (OCD) in ovaries is indicated (in ^m) for each female. stage and the synthesis of proteins in the adipose tissues (Table I). In excess antibody precipitations of all the sam- ples, no Vt-specific proteins were detected. Ovaries from the same females all produced Vt in vitro, at levels aver- aging 38% of the total de novo synthesized protein (Browdy etui. 1990; Table I). SAT proteins were separated in non-denaturating polyacrylamide gels. Total proteins were visualized with Coomassie Blue (Fig. 2A), while incorporation of radio- label into de novo synthesized SAT proteins was visualized by fluorography (Fig. 2B). Vt-specific immunological staining was performed on Western blots (Fig. 2C) and copper conjugated proteins by dithio-oxamide staining (Fig. 2D). Samples from previtellogenic and vitellogenic females and control males were run on each gel. Figure 2C shows that samples from SAT's of vitellogenic females stained positive for Vt. However, Vt was not visualized in the fluorographic exposures (Fig. 2B). One major ra- dioactive band is seen in the fluorography of all the sam- ples, including the male control. Based on the position of this band versus the position of hemocyanin (Fig. 2A), and on a positive reaction with the copper stain (Fig. 2D), the main de novo synthesized protein in adipose tissues appears to be hemocyanin or a copper containing protein with a similar electrophoretic mobility in non-denatur- ating polyacrylamide ge
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
