. Biophysical science. Biophysics. 540 Microscopy /29 : 2 light of one of these bright lines can pass, the transmitted light will spread out past the focal plane as if from a single line source. If, how- ever, light from two of the lines in the focal plane is allowed to pass the Image Plane (+g) [Magnified Real Image). Diffraction Order Focal Plane [+f) Lens [Objective) '—^—v—^j4 Grating [Object) (-/>) Figure 2. Grating and lens. This figure is used in derivation of the limit of resolution of a lens such as the objective lens of a microscope. screen, interference will occur. The interferenc
. Biophysical science. Biophysics. 540 Microscopy /29 : 2 light of one of these bright lines can pass, the transmitted light will spread out past the focal plane as if from a single line source. If, how- ever, light from two of the lines in the focal plane is allowed to pass the Image Plane (+g) [Magnified Real Image). Diffraction Order Focal Plane [+f) Lens [Objective) '—^—v—^j4 Grating [Object) (-/>) Figure 2. Grating and lens. This figure is used in derivation of the limit of resolution of a lens such as the objective lens of a microscope. screen, interference will occur. The interference pattern will have just the proper form to give rise to an image of the original grating, pro- vided that the latter was more than a focal distance from the lens. In fact, the image will occur at a distance q given by the lens formula 111 q P~f (2) In this, — p is the distance from the grating to the lens and / is the focal length of the lens. The plane at q is called the image plane of the objective. If the spacing of the grating is decreased, the number of bright lines in the focal plane will also decrease because sin 9 cannot exceed one. As this happens, the distance between the various diffraction orders increases. In the extreme cases, even the first-order diffraction line will disappear, leaving only the central line. When this occurs, no image of the grating can be formed by the Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ackerman, Eugene, 1920-. Englewood Cliffs, N. J. , Prentice-Hall
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