. dan bai zhi jie gou fen xi zhi bei jian ding yu wei liang ce xu. botany. è¡çåååº ä¸ºäºè·å¾å¥½çåç°çï¼åªè½ç¨ä¸ä¸ªå ¨èªå¨è¡çåç³»ç»ï¼å¦èªå¨ä¸æ ·å¨ï¼Promisn)ã è¦ç¨æ°é å¶çOPA溶液è¿è¡æ°¨åºé ¸è¡çåãè¿ç§æº¶æ¶²åºæ¯å¨é ä¸æ¬¡ï¼r:äº-2(rcã 1) æ°¨åºé ¸æ ååæå¾ åææ ·åç¨æ ·åç¼å²æ¶²ç¨éå°ç»æµåº¦100pmol/10|^Jã 2) æ°¨åºé ¸ååºç¨èªå¨ä¸æ ·å¨Promis IIå¨å®¤æ¸©ä¸èªå¨è¿è¡ï¼ååº1åéãè¯åä¸æ ·åæ¯ (v/v)为1 ï¼ 1 (æ¯ä¸ª10^d)ã 3) å离梯度æ¡ä»¶ï¼å¨1åéå ç±15% Bå°20% B;å¨12åéå ç±20% Bå°32% B;å¨ 10åéç±32% Bå°60% B; 3åéå ç±60% Bå°90% B; 2åéå ç±90%Bå°15%B; 15%Bç¸å½äº85%Aï¼ 20%Bç¸å½äº80%Aã æµé为åéï¼æ±ç®±æ¸©åº¦SOT ãè§å æ£æµï¼
. dan bai zhi jie gou fen xi zhi bei jian ding yu wei liang ce xu. botany. è¡çåååº ä¸ºäºè·å¾å¥½çåç°çï¼åªè½ç¨ä¸ä¸ªå ¨èªå¨è¡çåç³»ç»ï¼å¦èªå¨ä¸æ ·å¨ï¼Promisn)ã è¦ç¨æ°é å¶çOPA溶液è¿è¡æ°¨åºé ¸è¡çåãè¿ç§æº¶æ¶²åºæ¯å¨é ä¸æ¬¡ï¼r:äº-2(rcã 1) æ°¨åºé ¸æ ååæå¾ åææ ·åç¨æ ·åç¼å²æ¶²ç¨éå°ç»æµåº¦100pmol/10|^Jã 2) æ°¨åºé ¸ååºç¨èªå¨ä¸æ ·å¨Promis IIå¨å®¤æ¸©ä¸èªå¨è¿è¡ï¼ååº1åéãè¯åä¸æ ·åæ¯ (v/v)为1 ï¼ 1 (æ¯ä¸ª10^d)ã 3) å离梯度æ¡ä»¶ï¼å¨1åéå ç±15% Bå°20% B;å¨12åéå ç±20% Bå°32% B;å¨ 10åéç±32% Bå°60% B; 3åéå ç±60% Bå°90% B; 2åéå ç±90%Bå°15%B; 15%Bç¸å½äº85%Aï¼ 20%Bç¸å½äº80%Aã æµé为åéï¼æ±ç®±æ¸©åº¦SOT ãè§å æ£æµï¼340nmå¤æ¿åï¼å¨455nmå¤åå°ã å¾æ示为ççæ°¨åºé ¸æ ååå¨ä¸åæ¡ä»¶ä¸çå离ç»æï¼ 4) 注å°10^11å«100&0101æ°¨åºé ¸æ ååçæ ·åç¼å²æ¶²ï¼éæ°å¼å§æ¢¯åº¦ã 5) æ ¡æ-£HPLCç³»ç»ã 6) 注å°çº¦20pmolèç½æ°´è§£ç©ï¼å¼å§æ¢¯åº¦ã 7) 计ç®æ°¨åºé ¸çç»æã. 0 10 20 30 ä¿çæ¶é´ï¼åéï¼ å¾ SOpmolççæ°£åºé ¸æ ååç¨OPAæ±åè¡çååå¨åç¸C18æ±ä¸çå离ã P-SER:ç£·é ¸ä¸æ°¨é ¸ï¼Citr:çæ°£é ¸ï¼AAA:æ°£åºä¹äºé ¸ï¼ABA:æ°¨åºä¸é ¸ã NBD-C1æ±åè¡çå ææ ä¸ HPLCç³»ç»ï¼åOPAæ±åè¡çåï¼è§ä¸æï¼ ä¸åç¸C18æ±ï¼250mm X 4mm (Hypereil) am. HKl H3S- SSf- aa 0寸£X3 165. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original R. M. kan pu B. w
Size: 1785px × 1399px
Photo credit: © Central Historic Books / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcollectionbiodiversity, bookcontributorli, booksubjectbotany
