. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. DEVELOPMENTAL BIOLOGY 217. empty chorions Figure 1. Bovine serum albumin enhances chorion c\pan\ion 10 permit normal development and hutching of in vitro fertilised squid embryos, f Eiiflaeen-day embryos cultured in ' < BSA/MFS\\' permits i horion evpunsion mid normal development, id Twenty-one-day embryos, e. cultured in 'c \\'. Arrows indicate three empty chorions recently discarded h\ : Scale bars = 1000 fun. embryo culture enables us to treat cephalopod embryos with chem- ical agents, such
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. DEVELOPMENTAL BIOLOGY 217. empty chorions Figure 1. Bovine serum albumin enhances chorion c\pan\ion 10 permit normal development and hutching of in vitro fertilised squid embryos, f Eiiflaeen-day embryos cultured in ' < BSA/MFS\\' permits i horion evpunsion mid normal development, id Twenty-one-day embryos, e. cultured in 'c \\'. Arrows indicate three empty chorions recently discarded h\ : Scale bars = 1000 fun. embryo culture enables us to treat cephalopod embryos with chem- ical agents, such as lithium chloride (11) or retinoids (12). that have been shown to predictably alter development in other organ- isms, thereby enhancing our ability to work with and understand the embryos of this evolutionarily old and successful group of organisms. This work was supported by the Marine Biological Laboratory Associates Fellowships. The James A. and Faith Miller Memorial fund, and The Evelyn and Melvin Spiegel Fellowship Fund to Karen Crawford. Literature Cited 1. Brooks, \V. K. 1880. .4«;iiv. Mem. Boston Soc. ,\'at. Hist. 1-22. 2. Arnold, J. 1965. Biol. Bull. 128: 24-32. 3. Arnold, J. and L. D. Williams-Arnold. 1974. J. Embryol. Exp. Morp/iol. 31: 1-25. 4. Crawford, K. 1985. Biol. Bull. 169: 540 5. Crawford, K. 2000. Biol. Bull. 199: 207-208. 6. Crawford, K. 2001. Biol. Bull. 201: 251-252. 7. Klein, K., and L. A. Jaffe. 1984. Biol. Bull. 167: 518. 8. Sakai, M., and N. E. Brunetti. 1997. F/.V/I. Sci. 63(5): 664-667. 9 Callaerts. P., P. N. Lee. B. Hartmann, C. Farfan, D. W. Y. Choy, K. Ikeo, K-F. Fischbach, VV. J. Gehring, and H. Gert de Couet. 2002. Proc. AW//. Acad. Sci. USA 99: 20X8-2093. 10 Burbach. J. P. H., A. J. C. G. M. Hellemons. M. Hoekman, P. Grant, and H. C. Pant. 2001. Biol. Bull. 201: 252-254 11. Kao, K. V. Masui. and R. P. Elinson. 1986. Nature 322: 371-373. 12. Marshall. H., S. Nonchev, M. H. Sham, I. Muchamore, A. Lums- den, and R. Krumlauf. 1992. Natu
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