. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 16 P. S. GLAS ET Figures 1-6. Sicvimia ingenlis eggs. HE, hatching envelope: PVS. perivitelline space; ATA, 3-amino- 1,2,4-tnazole. Figure 1. With phase microscopy, eggs in artificial seawater show a distinctive retractile HE surrounding the PVS (*). The second polar body (pb) is visible within the PVS. Bar equals 100 jim. Figure 2. With transmission electron microscopy, eggs in artificial seawater show the jelly layer (JL) outside of the HE (HE). The PVS (*). separating the HE and oolemma (O). contains materials that m
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 16 P. S. GLAS ET Figures 1-6. Sicvimia ingenlis eggs. HE, hatching envelope: PVS. perivitelline space; ATA, 3-amino- 1,2,4-tnazole. Figure 1. With phase microscopy, eggs in artificial seawater show a distinctive retractile HE surrounding the PVS (*). The second polar body (pb) is visible within the PVS. Bar equals 100 jim. Figure 2. With transmission electron microscopy, eggs in artificial seawater show the jelly layer (JL) outside of the HE (HE). The PVS (*). separating the HE and oolemma (O). contains materials that may be added to the HE. Bar equals 1 jim. Figure 3. At higher magnification, the bilayered structure of the HE is apparent. The outer dense layer (DL) forms a smooth exterior while the inner flocculent layer (IF) appears to be incorporating more material from the PVS (*). Bar equals /im. Figure -4. Eggs in 3-amino-l,2,4-triazole (ATA) seawater have HEs (HE) that often collapse. The first polar body (pb) is visible outside of the collapsed HE. Bar equals 100 ^m. Figure 5. With transmission electron microscopy, eggs in ATA seawater show HEs (HE) that do not have the structural bilayered appearance of those in control eggs. The inner electron translucent layer is missing. The PVS (*) separates the oolemma (O) from the HE. Bar equals 1 pm. Figure 6. Higher magnification shows that the ATA-treated HEs are thinner and do not appear to have the flocculent inner layer attached to the dense layer (DL). Bar equals ^m. (DHTMR) (Whitaker et a/., 1991), no fluorescence was visible in the egg before HE elevation. Sperm attached to the egg surface were visibly fluorescent (Fig. 15a, b). A fluorescent band appeared in the cortex of the egg as the HE became visible (Fig. 16a, b). A thin, fluorescent outline of the HE appeared after HE elevation. The HE fluores- cence intensified briefly and remained in the region of the newly elevated HE until about 6 i min postspawn (Fig. 17a, b). By
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
