Practical nursing : a text-book for nurses . efully in order todistinguish one variety from another. Some of theimportant points that the bacteriologist has to con-sider are: (1) In what kind of media the organismsgrow best. (2) If they cause putrefaction or fermenta-tion or produce gases, acids, colored matter, or phos-phorescence in the media; if they liquefy the latter.(3) If, and by what, they can be stained. (4) Whetherthey require oxygen or not. (5) Whether they havethe power of independent movement. This can betold only by examining them under the microscope.(6) Their shape and size. (7
Practical nursing : a text-book for nurses . efully in order todistinguish one variety from another. Some of theimportant points that the bacteriologist has to con-sider are: (1) In what kind of media the organismsgrow best. (2) If they cause putrefaction or fermenta-tion or produce gases, acids, colored matter, or phos-phorescence in the media; if they liquefy the latter.(3) If, and by what, they can be stained. (4) Whetherthey require oxygen or not. (5) Whether they havethe power of independent movement. This can betold only by examining them under the microscope.(6) Their shape and size. (7) Whether they developenzymes. (8) The results following their injectioninto animals, as rabbits and guinea-pigs. (9) Also, Bacteriology 7i there are different tests used for diagnostic purposes;familiar examples are the Widal and the Wassermanntests. The former will be described on page 76. Necessary Precautions in Laboratory Work.—It can be easily understood that if accurate results areto be obtained in laboratory work the greatest care. Fig. 2. must be taken to have, and keep, everything thatcomes in contact with the specimen being examinedperfectly sterile. For this reason slides1 and coverglasses2 are handled with sterile forceps rather than thefingers; when transferring media from a test-tube to aPetri dish, the tube and dish are held as shown inFig. 2 to avoid infection from the air; when inoculat-ing media in test-tubes the neck of the tubes is held 1 Small pieces of glass on which matter for inspection under themicroscope is spread. 2 Very small and thin pieces of glass used to cover the matteron the slide. 72 Practical Nursing in a flame after the removal and before the reinsertionof the sterile cotton stoppers. The stoppers are heldby the upper end as shown in Fig. 3; the part whichwill be reinserted in the tube must not touch position in which the tubes are held is shown inthe same figure. A platinum wire mounted in a glassor metal handle is often use
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