Examination of the urine; a manual for students and practitioners . Hellers the authors experience with this reagent, which hasbeen extensive, its delicacy has been found rather con-fusing clinically. It shows a double or triple ring, the lowestand densest of which is due to albumin, the upper to nucleo-albumin or urates, or both. Urine which does not show aring with Hellers test occasionally shows a faint reaction,especially on standing, withRoberts test. Unless the lower ring is sharply de-fined, dense, and appearsalmost instantly, clinical al-buminuria cannot safely bediagnosticated
Examination of the urine; a manual for students and practitioners . Hellers the authors experience with this reagent, which hasbeen extensive, its delicacy has been found rather con-fusing clinically. It shows a double or triple ring, the lowestand densest of which is due to albumin, the upper to nucleo-albumin or urates, or both. Urine which does not show aring with Hellers test occasionally shows a faint reaction,especially on standing, withRoberts test. Unless the lower ring is sharply de-fined, dense, and appearsalmost instantly, clinical al-buminuria cannot safely bediagnosticated with Robertstest. It is most convenientlyemployed in the simple glassinstrument known as thehorismascope or albumo-scope (Fig. 6). The urine ispoured into the large endof the tube, and Robertssolution is then poureddown the small funneluntil the level of the solu-tion reaches about the center of the black background in the larger tube. The ringsare seen very distinctly against this background. Theinstrument can also be used with Hellers and with manyother Fig. 6.—The horismascope. 66 EXAMINATION OF THE URINE Mercuric Chlorid Test (Spiegler).—The reagent con-sists of 8 gm. of mercuric chlorid, 4 gm. of tartaric acid, and20 gm. of glycerin in 200 cc. of distilled water. It is usedby the contact method, like Hellers test. This test reactswith globulin and albumoses, but not with peptone. Spieg-lers test has recently been modified by Jolles, with theresult of rendering it much more delicate. Jolles recom-mends HgCl2, 10 grams; succinic acid, 20 grams; sodiumchlorid, 10 grams; water, 500 cc. A very sharp white ringis produced by albumin. The urine must first be filteredand acidified with acetic acid and filtered again to removenucleo-albumin. Salicylsulphonic Acid Test (Sulphosalicylic Acid Test,Roch and MacWilliams).—Add 1 or 2 drops of a sat-urated solution of the reagent, or more if the urine isalkaline, to about 20 drops of the urine in a small test-tube. On shaki
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