. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 292 B. CHANCE, L. SMITH, L. CASTOR VOL. 12 (1953) + .030 _ +.020 +.010 the spectrum representing the difference between the reduced and oxidized forms of the respiratory pigments of 5. albus illustrated by the trace labelled "reduced" in Fig. 2. And the trace labelled "reduced + CO" represents the difference between the CO compounds and the reduced forms of the respiratory pigments. In this case one observes a distinct peak at
. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 292 B. CHANCE, L. SMITH, L. CASTOR VOL. 12 (1953) + .030 _ +.020 +.010 the spectrum representing the difference between the reduced and oxidized forms of the respiratory pigments of 5. albus illustrated by the trace labelled "reduced" in Fig. 2. And the trace labelled "reduced + CO" represents the difference between the CO compounds and the reduced forms of the respiratory pigments. In this case one observes a distinct peak at 416 mju. and a trough at 432 m/x, as contrasted to the peak and trough of the corresponding spectrum for the yeast cells which lie at 430 and 445 mju, respectively. Similar studies can be carried out for S. albus in the visible region of the spectrum and peaks at 535 and 570 mjLt are reported by Smith^^. Fig. 2 when compared with Fig. 3B of refer- ence 9 shows that cultures of 5. albus may be treated so as to increase considerably their relative content of this CO-binding pigment. E « S o o. O. Fig. 2. The absorption difference spec- tra for a culture oi Staphylococcus albus obtained by means of the apparatus of Fig. I. The trace labelled "reduced" represents the differences between the reduced and oxidized cytochromes and the trace labeled "reduced -|- CO" represents the difference between the CO compound and the reduced form. Similar data can readily be obtained in the visible region (0-37). Photodissociation difference spectra Because their experimental conditions were in- appropriate, Keilin and Hartree were not able to demonstrate the photochemical dissociation of the CO compound of cytochrome a^. We have recently been able to accomplish this by a differential spectrophotometric method that is suitable for the observation of changes in absorption due to the photodissociation reaction within the respiring cell. This experiment is cons
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