. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 198 OLA BODVAR REITE with Ringer's solution and a drop spread on a slide of the acrylic resin "Perspex" and covered with a coverslip. A small piece of solid carbon dioxide in tinfoil wras then placed at the edge of the coverglass, and the freezing of the diluted blood was watched through a Leitz Ortholux microscope with Leica camera for photomicrography. Magnifications between 40 X and 200 X were used. A thin layer of glycerol on the coverglass facilitated observation by avoiding condensation of
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 198 OLA BODVAR REITE with Ringer's solution and a drop spread on a slide of the acrylic resin "Perspex" and covered with a coverslip. A small piece of solid carbon dioxide in tinfoil wras then placed at the edge of the coverglass, and the freezing of the diluted blood was watched through a Leitz Ortholux microscope with Leica camera for photomicrography. Magnifications between 40 X and 200 X were used. A thin layer of glycerol on the coverglass facilitated observation by avoiding condensation of water. RESULTS With the piece of solid carbon dioxide in place, ice formation in the diluted blood began immediately. The ice crystal front grew into the preparation and. FIGURE 1. The ice front is advancing through diluted blood from the congo eel. Shrinkage of the red blood cells starts as soon as they are reached by the spear-shaped ice crystals. The volume occupied by ice compared to the volume of the fluid space among the ice crystals indicates the portion of water withdrawn from solution. Magnification 100 X. water was withdrawn from solution. Shrinkage of the red blood cells started when they were reached by the ice front (Fig. 1) and continued concurrent with the decrease of fluid space among the ice crystals. At first ice crystals appeared exclusively in the suspending medium. Behind the ice front, however, ice crystals formed suddenly within one after the other of the shrunken cells. The crystals were so small that the light was scattered and the cell interior became opaque and appeared quite black (Fig. 2, A). When a definite extent of extracellular ice formation and thereby a certain degree of cooling was reached, the phenomenon would occur at various distances behind the ice front. The spontaneous intracellu- lar freezing took place only as long as the ice front was advancing. The opaque. Please note that these images are extracted from scanned page images that ma
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
