. DNA ji RNA ji ben shi yan ji shu. botany. PCRååºå产ç©å离 1) PCRå¼ç©ï¼ç¨éåæçå¯¡æ ¸è·é ¸å¼ç©è³æµåº¦ä¸º10 mmol/L (åè§æ³¨é1)ï¼ - 20â¬ä¿ å-ãå¼ç©è®¾è®¡æ¯PCRååºæåçå ³é®ï¼åè§æ³¨é2å3)ã 2) 10 x PCRç¼å²æ¶²ï¼100 mmol/L Tris-HCK pH (25å¸ï¼ï¼500 mmol/L KC1ï¼ 15 mmol/L MgCl2, (w/v)æè¶ãäº50å¸ä¸-å¾ æè¶å å溶解åè¿æ»¤é¤èï¼äº -20t:ä¿å-ã 3) dNTPæ··åç©ï¼æº¶äºè¸é¦æ°´ï¼æ¯ä¸ç§dNTPçæµåº¦ä¸º2 mmol/Lã dNTPæ¯æ¶²å¯ä»å ¬ å¸è´-å¾ï¼å¦Pharmacia (Piscataway, NJ)ã Boehringer (Indianapolis, IN)ãæ们åç° è¿äºäº§åæ¢æ¹ä¾¿åå¯é ã-201ï¼ä¿å-ã 4) ç¿ç©æ²¹ã 5) T^DNAèåé ¶ï¼5 U/plï¼ _20t:ä¿å-ã 6) PCRç»æ-¢æ··åç©ï¼25% Ficoll 4
. DNA ji RNA ji ben shi yan ji shu. botany. PCRååºå产ç©å离 1) PCRå¼ç©ï¼ç¨éåæçå¯¡æ ¸è·é ¸å¼ç©è³æµåº¦ä¸º10 mmol/L (åè§æ³¨é1)ï¼ - 20â¬ä¿ å-ãå¼ç©è®¾è®¡æ¯PCRååºæåçå ³é®ï¼åè§æ³¨é2å3)ã 2) 10 x PCRç¼å²æ¶²ï¼100 mmol/L Tris-HCK pH (25å¸ï¼ï¼500 mmol/L KC1ï¼ 15 mmol/L MgCl2, (w/v)æè¶ãäº50å¸ä¸-å¾ æè¶å å溶解åè¿æ»¤é¤èï¼äº -20t:ä¿å-ã 3) dNTPæ··åç©ï¼æº¶äºè¸é¦æ°´ï¼æ¯ä¸ç§dNTPçæµåº¦ä¸º2 mmol/Lã dNTPæ¯æ¶²å¯ä»å ¬ å¸è´-å¾ï¼å¦Pharmacia (Piscataway, NJ)ã Boehringer (Indianapolis, IN)ãæ们åç° è¿äºäº§åæ¢æ¹ä¾¿åå¯é ã-201ï¼ä¿å-ã 4) ç¿ç©æ²¹ã 5) T^DNAèåé ¶ï¼5 U/plï¼ _20t:ä¿å-ã 6) PCRç»æ-¢æ··åç©ï¼25% Ficoll 400, 100 mmol/L EDTA, (w/v)溴é èã 7) ç¼èç³åè¶ï¼å¨TAE(50 x TAE:242 g Trisç¢±ï¼ mlå°éé ¸ï¼100 ml mol/L EDTA, pH )æTBE (10XTBE: 121 g Tris, 55gç¡¼é ¸ï¼ g/L EDTA)ä¸-ç åçµæ³³çº§çç¼èç³ï¼ç¼æ ¢ç ®æ²¸ï¼å¯ç¨å¾®æ³¢çï¼ãå·å´è³ææ¡ä¸ç«æ¶åå ¥è¶æ¨¡ãå¨ 100Vå·¦å³è·å°ååè¶ï¼ç¨ç»æ-¢ç¼å²æ¶²ä¸-çææä½ä¸ºè¿ç§»ç¤ºè¸ªåï¼åè§æ³¨é4)ã 8) é ï¼è¶ 纯度çé å¨TEä¸-平衡饱åã4"Cä¿å-ã 9) 氯仿ï¼æ29:1ä¸å¼æéæ··åã ((mn\\ dnaåæ ç¨Taqèåå¯ åå¼ç©å¯¹è¿è¡ PCRæ©å¢ T-è½½ä½ PCR产ç©. å«ææå ¥ç段 çéç»è´¨ç² å¾ç¨T-è½½ä½å éPCR产ç©ç示æå¾ã £coRVå¹³æ« ç«¯çæ¶å. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original A. J. ha wu de. ke xue chu ban she
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