. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 266 JOHN DAVISON respirometer. The animals were blotted with filter paper and weighed fresh follow- ing the determination. No sex differences were observed. Oxygen consumption is expressed as cc. O2 fresh weight, and gas volumes are reduced to stand- ard temperature and pressure (760 mm. Hg and 0° C.). No seasonal influence on breeding is apparent in the population, and the metab- olism measurements reported here were obtained between September and March. Determination of cell surface The animal was weighed, decapita
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 266 JOHN DAVISON respirometer. The animals were blotted with filter paper and weighed fresh follow- ing the determination. No sex differences were observed. Oxygen consumption is expressed as cc. O2 fresh weight, and gas volumes are reduced to stand- ard temperature and pressure (760 mm. Hg and 0° C.). No seasonal influence on breeding is apparent in the population, and the metab- olism measurements reported here were obtained between September and March. Determination of cell surface The animal was weighed, decapitated and the abdomen removed. A small portion of abdominal flexor muscle was dissected out and teased apart on a glass -- o or i CM 0 .05 <J o .01 SLOPE = 0 SLOPE— 25 C. BODY WEIGHT CMS. H 1- H h .001 .010 .100 FIGURE 1. Resting oxygen consumption in Procambarus alleni plotted against the body weight in grams. Both ordinate and abscissa are in log form. The equation of the experi- mentally determined line is : cc. O2 = (W)-°'24. 25° C. slide. The teased muscle was then stained with acetocarmine in 45% acetic acid. It is necessary to stain the muscle cell nuclei in order to easily identify single fibers. The preparation was observed under a suitable power of the com- pound microscope. About 20 fibers were chosen at random, and the fiber diameters projected on paper by means of the camera lucida. A known linear dimension was projected from a stage micrometer, and the mean fiber diameter determined. For a sample of 20 fibers, the standard deviation was found to be about 20% of the mean. Assuming the fibers approximate long cylinders without free ends, their. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Bi
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
