. Carnegie Institution of Washington publication. Changes in Salinity and Effects on Regeneration, etc. 87 sea-water, nor on the other hand was there any regeneration in solutions 133 or more per cent, with one exception to be noted later. Though regener- ation occurred within these limitations, the character of the resulting regenerated arms was not the same. Normal pyramid-shaped arms with characteristic mouths and oral filaments were developed in the median solutions only, i. e., 75 to 105 per cent inclusive. In the 70 per cent solution Fig. I.—Thin line represents regen- 5 eration after 14
. Carnegie Institution of Washington publication. Changes in Salinity and Effects on Regeneration, etc. 87 sea-water, nor on the other hand was there any regeneration in solutions 133 or more per cent, with one exception to be noted later. Though regener- ation occurred within these limitations, the character of the resulting regenerated arms was not the same. Normal pyramid-shaped arms with characteristic mouths and oral filaments were developed in the median solutions only, i. e., 75 to 105 per cent inclusive. In the 70 per cent solution Fig. I.—Thin line represents regen- 5 eration after 14 days: thick line ^ 24 days; broken line 30 days. 3. 153 142 133 125 117 111 105 100 95 90 85 80 75 70 65 60 55 50 45 40 the mouths were considerably reduced in number, while the number of oral filaments was scarcely affected. In the 65 per cent solution there were very few mouths differentiated. In the 60 and 55 per cent solutions practically no mouths were formed and the oral filaments tended to clump together. Similarly, beginning with the iii per cent solution, of the concentrated series, the arms were increasingly atypic. In this solution some arms were normal, others clumped as in the diluted solutions. In the 117 per cent solution, fewer mouths were differentiated and greater crowding of the oral filaments occurred. In the 125 per cent solution no mouths and only very few filaments were differentiated. It is then clear that while regeneration took place in a wide range of solutions, normal regeneration occurred within much narrower limits, namely, 75 to 105 per cent solutions. Corroborative results were obtained from measurement of regenerated arms. Figure i and table i clearly show that when the average regeneration in sea-water is taken as the norm, then the average regeneration in iii to 80 per cent solutions is norm or supernorm, varying from to mm. On either side of these limits there is a rapid decline, sharper in the more concentrated solutions, far less
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