. The American journal of tropical medicine. These were rounded,not over 2 mm. in diameter and moruliform in shape. Whenpressed underneath a cover slip and examined microscopically,they appeared to be composed of aggregations of smaller bodies,at the periphery of which there were observed a large number ofshort clavate projections or knobs (fig. 12). The granules weresoft in consistency and smeared out readily. \Yhen stained byGrams method, the smear is seen to be composed of a Gramnegative material, mostly in rectangular masses of a uniformsize, together with a Umited number of spatulate bodi
. The American journal of tropical medicine. These were rounded,not over 2 mm. in diameter and moruliform in shape. Whenpressed underneath a cover slip and examined microscopically,they appeared to be composed of aggregations of smaller bodies,at the periphery of which there were observed a large number ofshort clavate projections or knobs (fig. 12). The granules weresoft in consistency and smeared out readily. \Yhen stained byGrams method, the smear is seen to be composed of a Gramnegative material, mostly in rectangular masses of a uniformsize, together with a Umited number of spatulate bodies, with MYCETOMA IN NORTH AMERICA 259 a dense oval Gram positive center and a Gram negative outerwall which presents the spatulate shape (Fig. 13). These bodiesare also acid fast. They correspond to the solitary conidia-spores seen in cultures, and to the spatulate projections fromthe periphery of the granule. Sections of a grain show it to be a dense homogeneous networkof mjTelial threads which cannot be differentiated into zones().. Fig. 8. Giant Colonies of Allescheria Boydii on Sabourauds at 37°C. for Three Weeks Some of the above granules were washed by passing throughseveral changes of sterile saline, teased out into fragments andthe fragments planted onto the surface of slants and into thedepths of plain agar, Sabourauds agar, Huntoons agar anddextrose agar. Two sets of each were prepared, one inoculatedat 37°C\ and the other at room temperature. By forty-eighthours, each granule on the surface of a tube of slanted media,regardless of the temperature of incubation, was surrounded by 200 MARK F. BOYD AND EARL D. CRUTCHFIELD a delicate fringe of mycelium, which rapidly extended. Thedeep implantation did not show growth until the fifth day, whichwas only evident from those granules not submerged over 5 the surface. Those planted at greater depths did not every instance growth radiated from the implanted granuleand occurred at no other
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Keywords: ., bookcentury1900, bookdecade1920, booksubjecttropica, bookyear1921
