. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 2B 2A. 2C FIGURE 1. Two main types of hemocyte can be identified in the blood of Uca: granulocytes (g) and hyaline cells (h). In Figure 1A these hemocytes aggregate in great numbers near the epidermis and soft cuticle (cut) of a crab fixed in a buffered glutaraldehyde-salt fixative. Granulocytes and hyaline cells appear scattered within the eosinophilic serum which contains numerous granules (arrow). Certain granules exhibit basophilia; others exhibit acidophilia. Az-Eo, pH MEL, melanophore. X430. Figure IB shows the po
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 2B 2A. 2C FIGURE 1. Two main types of hemocyte can be identified in the blood of Uca: granulocytes (g) and hyaline cells (h). In Figure 1A these hemocytes aggregate in great numbers near the epidermis and soft cuticle (cut) of a crab fixed in a buffered glutaraldehyde-salt fixative. Granulocytes and hyaline cells appear scattered within the eosinophilic serum which contains numerous granules (arrow). Certain granules exhibit basophilia; others exhibit acidophilia. Az-Eo, pH MEL, melanophore. X430. Figure IB shows the positive reaction for arginine in the granulocytes (g) and the negative reaction in the hyaline cells (h). NQS. X600. Figure 1C demonstrates reducing substances in small (immature?) granulocytes (arrows) found deep within the hemocoel. Ferric ferricyanide. mu, muscle. X600. FIGURE 2. Besides hyaline cells and granulocytes, a third type of hemocyte (intermediate or transitional type) can be seen within the hemocoel. In Figure 2A a specimen fixed in formalin:acid:salt during late intermolt, the intermediate type (transitional cystocyte) hemocyte (i, arrow) approximates the size of the hyaline cell (h) and exhibits tiny unstained granules within a less extensive cytoplasm than the granulocytes (g). Az-Eo, pH X900. Figure 2B shows intermediate cells (arrows) releasing tiny proteinaceous granules which have azo-coupled with DAS. The hyaline cell (h) contains diphenols. The hemocytes appeared in a specimen fixed 10-15 hours postecdysis in buffered formalin-salt. X600. Figure 2C represents a diagrammatic interpretation of the intermediate cells rupturing and releasing their granules, thereby forming a cytoplasmic network which may function in clotting. 761. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
