. Bulletin of the Department of Agriculture. Agriculture; Agriculture. BULLETIN 629, U. S. DEPARTMENT OF AGEICULTUEE. them on the previously moistened leaf to be inoculated. When all leaves in a pot were inoculated they were sprayed at once with an atomizer and placed in the moist chamber shown in figure 1, where they were allowed to remain 48 hours. These moist chambers, which will hold about forty 4-inch pots, cost less than $10 for four. No trouble was experienced from the leaves burning or turning yellow, and almost 100 per cent of the inoculations were successful. Inoculations of old
. Bulletin of the Department of Agriculture. Agriculture; Agriculture. BULLETIN 629, U. S. DEPARTMENT OF AGEICULTUEE. them on the previously moistened leaf to be inoculated. When all leaves in a pot were inoculated they were sprayed at once with an atomizer and placed in the moist chamber shown in figure 1, where they were allowed to remain 48 hours. These moist chambers, which will hold about forty 4-inch pots, cost less than $10 for four. No trouble was experienced from the leaves burning or turning yellow, and almost 100 per cent of the inoculations were successful. Inoculations of older plants were made in the order of heading of the varieties, beginning on April 10 and continuing to May 9, 1916. There were 12 to 18 plants in each series. The stem-rust spores were alwaj^s placed on the sheath inclosing the emerging panicle, while the inoculations with crown rust were made on the uppermost leaf blade. As with the seedlings, the inoculated plants were sprayed with an atomizer to insure the presence of a film of water and then kept in the moist chamber for two * days. A special large glass-topped galvan- ized-iron moist chamber was made, holding eight- een 5-inch pots and allow- ing the tallest plants to re- main upright (fig. 2). The incubation period for the two rusts was ap- proximately the same. Generally, though not al- ways, the uredinia of the stem rust appeared first. Cool temperatures seemed to lengthen the incubation period, for during November the house was cooler than during the succeeding months and the uredinia during this time were noticeably slower in appearing. Other factors, such as light, also may have affected the results. Notes on the appearance of flecks usually were made in 7 to 9 days, and those on the formation of uredinia after a period of not longer than 12 days. Further notes usually were taken on the quantity and character of infection. No counts of uredinia were made; nor should too much emphasis be placed on whether or not all th
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