. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FATTY ACID ABSORPTION IN H. DIMINUTA 83 2«r. 8 mM acetate FIGURE 4. The uptake of 14C-acetate at pH and as a function of acetate concentration. Each point is a mean of 8 determinations. Analysis of the data on acetate uptake in terms of Michaelis-Menten kinetics allowed an evaluation of Kt (equivalent to Michaelis' constant) and V,nax (extrap- olated maximum velocity). In the concentration range to mM acetate, Kt and Vmax were mM and //., respectively, when the data were not corrected f
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FATTY ACID ABSORPTION IN H. DIMINUTA 83 2«r. 8 mM acetate FIGURE 4. The uptake of 14C-acetate at pH and as a function of acetate concentration. Each point is a mean of 8 determinations. Analysis of the data on acetate uptake in terms of Michaelis-Menten kinetics allowed an evaluation of Kt (equivalent to Michaelis' constant) and V,nax (extrap- olated maximum velocity). In the concentration range to mM acetate, Kt and Vmax were mM and //., respectively, when the data were not corrected for diffusion. After correction, Kt was mM and Vmax was The uptake of sodium acetate with respect to time is shown in Figure 3. Fol- lowing 40 minutes incubation in a substrate concentration of mM the amount of radioactive material extracted from the worm indicated an internal acetate con- centration in the worm water of mM. Preliminary chromatography indicated that about 60% of the radioactivity extracted was unaltered acetate, but further study would be required to determine more precisely the proportion of the radio- activity representing metabolically unaltered acetate. However, it may be sug- gested that mediated acetate uptake is a facilitated diffusion rather than active transport. TABLE I Effects of pH on acetate uptake and on propionate -inhibition of acetate uptake. Acetate concentration mM; propionate concentration 4 mM Uptake (m/ ± ) pH Inhibition Acetate alone Acetate + propionate 161 ± 134 ± 17 147 ± 102 ± 31 139 ± 64 ± 54 135 ± 53 =t 61 129 zt 39 db 70. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Wo
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
