. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 342 GREENBERG, KUNKEL, AND STUART .9 .6 A 550 .3 B C. j_ 100 35 Mo I wt 25 x 10~3) FIGURES 6. Purified yolk protein from female neotenic reproductives, separated by SDS- polyacrylamide gel electrophoresis ( acrylamide). Molecular weights represent those of the standards phosphorylase a, 100,000; pepsin, 35,000; trypsin, 25,000; and lysosome, 14,300 Daltons. The molecular weights of the three major subunits were estimated to be A, 112,000; B, 89,000 and C, 71,000 Daltons. since, as in other insects, they were found o
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 342 GREENBERG, KUNKEL, AND STUART .9 .6 A 550 .3 B C. j_ 100 35 Mo I wt 25 x 10~3) FIGURES 6. Purified yolk protein from female neotenic reproductives, separated by SDS- polyacrylamide gel electrophoresis ( acrylamide). Molecular weights represent those of the standards phosphorylase a, 100,000; pepsin, 35,000; trypsin, 25,000; and lysosome, 14,300 Daltons. The molecular weights of the three major subunits were estimated to be A, 112,000; B, 89,000 and C, 71,000 Daltons. since, as in other insects, they were found only in female reproductives, appeared in the hemolymph prior to oocyte growth, and became the major proteins in the mature oocytes. The hemolymph vitellogenins are, furthermore, immunologically identical to TEAE-cellulose purified vitellin obtained from eggs. The hemolymph vitellogenins are also immunologically identical in both the neotenic and adult reproductives of Z. angusticollis. Thus, there do not appear to be any specific differences in the vitellogenins produced by the two reproductive sub-castes of this social insect species. This is similar to what has been found for the honey bee Apis mellifera where the female specific protein of workers and queens is im- munologically identical (Rutz and Liischer, 1974). The data demonstrate that in neotenic reproductives of Z. angusticollis, vitello- genin is first produced, or at least released into the hemolymph, seven to nine days. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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