. Recent research on foliage diseases : conference proceedings : Carlisle, Pennsylvania, May 29-June 2, 1989. Leaves Diseases and pests United States Congresses. Figure 1.—In vitro technioque used to study infection of green ash by Gnomoniella fraxini. A. Plastic petri dish (100 mm diam.) containing 2% water agar (4 mm deep) with wells cut using a sterile cork borer (18 mm diam.) and leaf discs of corresponding size being placed into wells with a sterile forceps. B. Arrangement of ash leaf discs in wells in water agar. Arrow indicates 4 mm diam. aluminum rod the tip of which was heated and tou
. Recent research on foliage diseases : conference proceedings : Carlisle, Pennsylvania, May 29-June 2, 1989. Leaves Diseases and pests United States Congresses. Figure 1.—In vitro technioque used to study infection of green ash by Gnomoniella fraxini. A. Plastic petri dish (100 mm diam.) containing 2% water agar (4 mm deep) with wells cut using a sterile cork borer (18 mm diam.) and leaf discs of corresponding size being placed into wells with a sterile forceps. B. Arrangement of ash leaf discs in wells in water agar. Arrow indicates 4 mm diam. aluminum rod the tip of which was heated and touched to leaf discs for wounding treatment. A burn-wounded leaf disc is shown below the rod tip. C. Green ash leaf discs nine days after inoculation. Nonwounded disc (L) remained healthy with normal green color (small spots are water droplets on the disc surface). Wounded noninoculated disc (C) shows necrotic tissue (arrow) resulting from original burn wound. Wounded disc inoculated with G. fraxini. (R) shows lesion developing beyond original burn wound. Arrow indicates lesion margin surrounded by chlorotic ring. 139. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Merrill, William; Ostry, Michael E; United States. Forest Service; International Union of Forestry Research Organizations. Working Party on Foliage Diseases. Washington, D. C. : U. S. Dept. of Agriculture, Forest Service
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