. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HERPOMYCES INFECTIONS IN COCKROACHES 211 but some have eight. Clearly there are eight in //. stylopy(/ae (Figs. 7 and 8). The asci are a fat cigar-shape and measure about 12 X 60 ^,, those used for the photographs seeming to be broader because somewhat flattened by cover-glass pressure to bring the spores into one focal plane. An apparently new point is that in this species the asci do not disintegrate to liberate spores within the perithecia. Perithecia of living plants teased in a drop of water liberate both spore-filled a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HERPOMYCES INFECTIONS IN COCKROACHES 211 but some have eight. Clearly there are eight in //. stylopy(/ae (Figs. 7 and 8). The asci are a fat cigar-shape and measure about 12 X 60 ^,, those used for the photographs seeming to be broader because somewhat flattened by cover-glass pressure to bring the spores into one focal plane. An apparently new point is that in this species the asci do not disintegrate to liberate spores within the perithecia. Perithecia of living plants teased in a drop of water liberate both spore-filled and empty asci (Figs. 8 and 9). The spore- filled asci were sometimes seen to liberate their spores one after another through. 10 I! FIGURE 7. Mature ascus with its eight spores after staining with the gram stain. From perithecium of H. stylopygae. Note that both spores and the ascus wall are gram positive. Bar 10 /". FIGURE 8. Mature ascus of H. stylopygae in water; somewhat compressed by coverglass pressure. Dark phase-contrast photomicrograph of living material. The pressure used to get all the spores into one focal plane has resulted in a rupture of the ascus wall and extrusion of material at the lower right of the figure (such rupture is not normal). Bar 10 M. FIGURE 9. Empty ascus or ascus ghost of H. stylopygae in water. Dark phase-contrast photomicrograph (not stained). Note contents, absence of any indication of rupturing or dis- integration (spores released through terminal pore), absence of any operculum, and apparently unitunicate condition. Bar 10 /J-. FIGURE 10. Electron micrograph of empty ascus ghost of H. stylopygae. Shows extreme thinness of dry ascus wall, and hence small amount of solid material therein, and no indication of more than a single layer. Bar 10 M. FIGURE 11. Living spores of //. stylopygae in water; teased from fresh perithecium. Note clustering of the spores. Bar 10 M. an aperture in the end as drying of the water began to exert cover-glass
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
